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TLR4 抗体 (AA 238-609)

This anti-TLR4 antibody is a 小鼠 单克隆 antibody detecting TLR4 in FACS, IHC, ELISA 和 IHC (p). Suitable for 人.
产品编号 ABIN334262
发货至: 中国

Quick Overview for TLR4 抗体 (AA 238-609) (ABIN334262)

抗原

See all TLR4 抗体
TLR4 (Toll-Like Receptor 4 (TLR4))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This TLR4 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

1E11
  • 抗原表位

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    AA 238-609

    特异性

    Recognizes human Toll-like Receptor 4 (TLR4).

    纯化方法

    Purified

    免疫原

    Recombinant protein corresponding to amino acids 238-609 of human Toll-like Receptor 4 expressed in E. coli.

    Type of Immunogen: Recombinant protein

    亚型

    IgG2a
  • 应用备注

    Approved: ELISA (1:2000 - 1:4000), Flo, IHC, IHC-P (5 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.Flow Cytometry: 3 μg/10e6 cellsOptimal dilutions to be determined by researcher.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    TLR4 (Toll-Like Receptor 4 (TLR4))

    别名

    TLR4

    背景

    Name/Gene ID: TLR4
    Family: Toll-like Receptor

    Synonyms: TLR4, CD284, CD284 antigen, Homolog of Drosophila toll, TLR-4, TOLL, ARMD10, Toll-like receptor 4, HToll

    基因ID

    7099

    UniProt

    O00206

    途径

    TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins
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