GZMB 抗体
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北京 101111
Quick Overview for GZMB 抗体 (ABIN330561)
抗原
See all GZMB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes human Granzyme B. Shows no cross-reactivity with rhGranzyme A, -H, rmGranzyme B, -C, -D, or -G.
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纯化方法
- Protein G purified
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免疫原
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NS0-derived rhGranzyme B.
Type of Immunogen: Recombinant protein -
亚型
- IgG2a
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anti-Granzyme B (GZMB) (AA 21-247) antibody
GZMB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Granzyme B (GZMB) (AA 19-247) antibodyGZMB 适用: 人 WB, IHC, ICC, IF (p), mIF 宿主: 兔 Monoclonal BLR022E unconjugated Recombinant Antibody
anti-Granzyme B (GZMB) (AA 73-187) antibodyGZMB 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal GZMB-3014 unconjugated
anti-Granzyme B (GZMB) (AA 21-130) antibodyGZMB 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Granzyme B (GZMB) (AA 1-247) antibodyGZMB 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Granzyme B (GZMB) (AA 21-247) antibodyGZMB 适用: 人 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 4E4 unconjugated
anti-Granzyme B (GZMB) antibodyGZMB 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Granzyme B (GZMB) (AA 73-187) antibodyGZMB 适用: 人 IHC 宿主: 小鼠 Monoclonal GZMB-3056 unconjugated
anti-Granzyme B (GZMB) (AA 21-247) antibodyGZMB 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2G12G7 unconjugated
anti-Granzyme B (GZMB) antibodyGZMB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CLB-GB11 unconjugated
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应用备注
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Approved: ELISA, Flo, IHC, WB
Usage: Western Blot, Flow Cytometry, Immunohistochemistry, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human Granzyme B. Using a colorimetric detection system, the detection limit for rhGranzyme B is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was tested for flow cytometry using NK-92 cells. For intracellular staining to detect human Granzyme B, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. Following a 30 minute incubation, cells should be washed with 0.1 % saponin prior to addition of a secondary developing reagent. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis. Immunohistochemistry: This antibody was used at a concentration of 8-25 μg/mL with appropriate secondary reagents to detect human Granzyme B in paraformaldehyde-fixed human PBMC. For chromogenic detection of labeling, the use of Cell and Tissue Staining Kits is recommended. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Granzyme B. The detection limit for rhGranzyme B is approximately 5 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
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- GZMB (Granzyme B (GZMB))
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别名
- GZMB / Granzyme B
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背景
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Name/Gene ID: GZMB
Subfamily: Serine S1
Family: Protease
Synonyms: GZMB, CCPI, CGL-1, Cathepsin G-like 1, CSP-B, CTLA-1, CTLA1, CGL1, CSPB, Fragmentin 2, GRB, HLP, Human lymphocyte protein, Fragmentin-2, SECT, Lymphocyte protease, T-cell serine protease 1-3E, C11, CTSGL1, Cytotoxic serine protease B, Granzyme B, Granzyme-2 -
基因ID
- 3002
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UniProt
- P10144
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
-
