DYKDDDDK Tag 抗体 (C-Term)
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北京 101111
Quick Overview for DYKDDDDK Tag 抗体 (C-Term) (ABIN329107)
抗原
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- DYKDDDDK
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特异性
- Recognizes the FLAG epitope tag fused to the amino-terminus of targeted proteins. Reacts with all amino-terminal FLAG tagged proteins so far tested. No reactivity is seen against fusion proteins containing the FLAG epitope tag at the carboxy-terminus. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.
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纯化方法
- Immunoaffinity purified
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免疫原
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FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the carboxy terminal end to facilitate coupling.
Type of Immunogen: Synthetic peptide
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应用备注
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Approved: ELISA (1:10000 - 1:50000), IHC, WB (1:1000 - 1:5000)
Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:10000-1:50000. Western Blot: 1:1000-1:5000. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- DYKDDDDK Tag
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物质类
- Tag
抗原
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