IL17D 抗体 (AA 25-205)
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Quick Overview for IL17D 抗体 (AA 25-205) (ABIN327476)
抗原
See all IL17D 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 25-205
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特异性
- Recognizes mouse IL-17D. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rhIL-17D, rmIL-17, rmIL-17B, rmIL-17C, rmIL-17E or rmIL-17F.
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纯化方法
- Protein G purified
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免疫原
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Recombinant protein corresponding to aa25-205 from mouse Interleukin 17D expressed in E. coli (NP_665836).
Type of Immunogen: Recombinant protein -
亚型
- IgG2a
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anti-Interleukin 17D (IL17D) antibody
IL17D 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17D (IL17D) (AA 1-202) antibodyIL17D 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17D (IL17D) (AA 76-274) antibodyIL17D 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17D (IL17D) antibodyIL17D 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17D (IL17D) (Internal Region) antibodyVerified IL17D 适用: 人 ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 17D (IL17D) (AA 16-202) antibodyIL17D 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17D (IL17D) (AA 55-65) antibodyVerified IL17D 适用: 人 ELISA, WB 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 17D (IL17D) (N-Term) antibodyIL17D 适用: 人, 小鼠, Cow, 豚鼠, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17D (IL17D) (N-Term) antibodyIL17D 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17D (IL17D) (AA 35-169) antibodyIL17D 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, Flo, WB
Usage: Western Blot, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect mouse IL-17D. Using a colorimetric detection system, the detection limit for rmIL-17D is approximately 5 ng/lane under non-reducing and reducing conditions. Use of this antibody under non reducing/reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using CD4+ splenic lymphocytes. For intracellular staining to detect IL-17D, cells must first be fixed and permeabilized with 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse IL-17D. The detection limit for rmIL-17D is approximately 1 ng/well. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 200 μL sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose.
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储存条件
- 4 °C,-20 °C
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储存方法
- Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
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- IL17D (Interleukin 17D (IL17D))
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别名
- IL17D
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背景
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Name/Gene ID: IL17D
Family: Interleukin
Synonyms: IL17D, IL-27, Interleukin 27, Interleukin-17D, Interleukin-27, IL-17D, IL-22, Interleukin 17D -
基因ID
- 53342
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NCBI登录号
- NP_665836
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UniProt
- Q8TAD2
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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