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IL17D 抗体 (AA 25-205)

IL17D 适用: 小鼠 ELISA, WB, FACS 宿主: 大鼠 Monoclonal unconjugated
产品编号 ABIN327476
发货至: 中国
  • 抗原 See all IL17D 抗体
    IL17D (Interleukin 17D (IL17D))
    抗原表位
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 25-205
    适用
    • 49
    • 13
    • 2
    • 2
    • 2
    • 1
    • 1
    小鼠
    宿主
    • 44
    • 4
    • 3
    • 2
    大鼠
    克隆类型
    • 46
    • 7
    单克隆
    标记
    • 30
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL17D antibody is un-conjugated
    应用范围
    • 31
    • 30
    • 15
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Flow Cytometry (FACS)
    特异性
    Recognizes mouse IL-17D. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rhIL-17D, rmIL-17, rmIL-17B, rmIL-17C, rmIL-17E or rmIL-17F.
    纯化方法
    Protein G purified
    免疫原
    Recombinant protein corresponding to aa25-205 from mouse Interleukin 17D expressed in E. coli (NP_665836).

    Type of Immunogen: Recombinant protein
    亚型
    IgG2a
    Top Product
    Discover our top product IL17D Primary Antibody
  • 应用备注
    Approved: ELISA, Flo, WB

    Usage: Western Blot, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect mouse IL-17D. Using a colorimetric detection system, the detection limit for rmIL-17D is approximately 5 ng/lane under non-reducing and reducing conditions. Use of this antibody under non reducing/reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using CD4+ splenic lymphocytes. For intracellular staining to detect IL-17D, cells must first be fixed and permeabilized with 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse IL-17D. The detection limit for rmIL-17D is approximately 1 ng/well.
    说明

    Target Species of Antibody: Mouse

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    200 μL sterile PBS
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS, 5 % trehalose.
    储存条件
    4 °C,-20 °C
    储存方法
    Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
  • 抗原
    IL17D (Interleukin 17D (IL17D))
    别名
    IL17D (IL17D 产品)
    背景
    Name/Gene ID: IL17D
    Family: Interleukin

    Synonyms: IL17D, IL-27, Interleukin 27, Interleukin-17D, Interleukin-27, IL-17D, IL-22, Interleukin 17D
    基因ID
    53342
    NCBI登录号
    NP_665836
    UniProt
    Q8TAD2
    途径
    Cellular Response to Molecule of Bacterial Origin
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