IL9 Receptor 抗体 (Extracellular Domain)
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- 抗原 See all IL9 Receptor (IL9R) 抗体
- IL9 Receptor (IL9R) (Interleukin 9 Receptor (IL9R))
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抗原表位
- Extracellular Domain
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This IL9 Receptor antibody is un-conjugated
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应用范围
- ELISA, Flow Cytometry (FACS), Functional Studies (Func), Neutralization (Neut)
- 特异性
- Recognizes human IL-9R. In direct ELISAs, this antibody does not cross-react with rhIL-4 R, rhIL-5 R, rhIL-5 R, rhIL-13 R 1, rhIL-13 R 2, or rmIL-9 R.
- 纯化方法
- Protein G purified
- 免疫原
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Sf21-derived rhIL-9 R extracellular domain.
Type of Immunogen: Recombinant protein - 亚型
- IgG1
- Top Product
- Discover our top product IL9R Primary Antibody
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- 应用备注
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Approved: ELISA, Flo, Func, Neut
Usage: Neutralization of bioactivity, Flow Cytometry, Direct ELISA. Neutralization of Human IL-9 Receptor-mediated bioactivity: The exact concentration of antibody required to neutralize the human cell surface IL-9 R mediated bioactivity is dependent on the IL-2 concentration as well as on the number and types of IL-2 receptors present on the cell surface (a function of cell type and culture conditions). The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-9 R mediated IL-9 response on a responsive cell line, at a specific IL-9 concentration. The ND50 for this lot of anti-human IL-9 R antibody was determined to be approximately 2-4 μg/mL for inhibiting the IL-9-dependent proliferation of MO7e cells (1 x 10^5 cells/mL) in the presence of 2 ng/mL of rhIL-9. Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human IL-9 R. The detection limit for rhIL-9 R is approximately 6 ng/well. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.
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- 抗原
- IL9 Receptor (IL9R) (Interleukin 9 Receptor (IL9R))
- 别名
- IL9R / CD129 (IL9R 产品)
- 背景
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Name/Gene ID: IL9R
Family: Interleukin
Synonyms: IL9R, IL-9 receptor, IL-9R, Interleukin 9 receptor, Interleukin-9 receptor, CD129, CD129 antigen - 基因ID
- 3581
- UniProt
- Q01113
- 途径
- JAK/STAT Signaling, Growth Factor Binding
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