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IL9 Receptor 抗体 (Extracellular Domain)

This anti-IL9 Receptor antibody is a 小鼠 单克隆 antibody detecting IL9 Receptor in ELISA, FACS, Func 和 Neut. Suitable for 人.
产品编号 ABIN327384
发货至: 中国

Quick Overview for IL9 Receptor 抗体 (Extracellular Domain) (ABIN327384)

抗原

See all IL9 Receptor (IL9R) 抗体
IL9 Receptor (IL9R) (Interleukin 9 Receptor (IL9R))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This IL9 Receptor antibody is un-conjugated

应用范围

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ELISA, Flow Cytometry (FACS), Functional Studies (Func), Neutralization (Neut)
  • 抗原表位

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    Extracellular Domain

    特异性

    Recognizes human IL-9R. In direct ELISAs, this antibody does not cross-react with rhIL-4 R, rhIL-5 R, rhIL-5 R, rhIL-13 R 1, rhIL-13 R 2, or rmIL-9 R.

    纯化方法

    Protein G purified

    免疫原

    Sf21-derived rhIL-9 R extracellular domain.

    Type of Immunogen: Recombinant protein

    亚型

    IgG1
  • 应用备注

    Approved: ELISA, Flo, Func, Neut

    Usage: Neutralization of bioactivity, Flow Cytometry, Direct ELISA. Neutralization of Human IL-9 Receptor-mediated bioactivity: The exact concentration of antibody required to neutralize the human cell surface IL-9 R mediated bioactivity is dependent on the IL-2 concentration as well as on the number and types of IL-2 receptors present on the cell surface (a function of cell type and culture conditions). The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-9 R mediated IL-9 response on a responsive cell line, at a specific IL-9 concentration. The ND50 for this lot of anti-human IL-9 R antibody was determined to be approximately 2-4 μg/mL for inhibiting the IL-9-dependent proliferation of MO7e cells (1 x 10^5 cells/mL) in the presence of 2 ng/mL of rhIL-9. Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human IL-9 R. The detection limit for rhIL-9 R is approximately 6 ng/well.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.
  • 抗原

    IL9 Receptor (IL9R) (Interleukin 9 Receptor (IL9R))

    别名

    IL9R / CD129

    背景

    Name/Gene ID: IL9R
    Family: Interleukin

    Synonyms: IL9R, IL-9 receptor, IL-9R, Interleukin 9 receptor, Interleukin-9 receptor, CD129, CD129 antigen

    基因ID

    3581

    UniProt

    Q01113

    途径

    JAK/STAT Signaling, Growth Factor Binding
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