Granulin 抗体
Quick Overview for Granulin 抗体 (ABIN325602)
抗原
See all Granulin (GRN) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Recognizes human GRN. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rmProgranulin.
-
纯化方法
- Protein G purified
-
免疫原
-
NS0-derived rhGRN.
Type of Immunogen: Recombinant protein -
亚型
- IgG2a
-
-
-
-
应用备注
-
Approved: ELISA (0.5 - 1 μg/mL), IP (25 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1.0 μg/mL, The detection limit for rhGRN is approximately 2 ng/well. Western Blot: 1-2 μg/mL, Using a colorimetric detection system, the detection limit for rhGRN is ~5 ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25 μg/mL to immunoprecipitate rhGRN from conditioned cell culture medium. The recovered rhGRN can be detected in Western Blots using an affinity-purified polyclonal antibody. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- 200 μL sterile PBS
-
浓度
- Lot specific
-
缓冲液
- Lyophilized from PBS, pH 8.0, 20 mM Tris, 300 mM sodium chloride, 5 % trehalose
-
注意事项
- Avoid repeat freeze-thaw cycles.
-
储存条件
- -20 °C
-
储存方法
-
Short term: -20°C
Long term: -20°C.
-
-
- Granulin (GRN)
-
别名
- GRN / Progranulin / Granulin
-
背景
-
Name/Gene ID: GRN
Family: Hormone
Synonyms: GRN, Acrogranin, CLN11, Epithelin precursor, GEP, gp88, Granulin, Granulin-epithelin, Granulins, PC cell-derived growth factor, PCDGF, PEPI, PGRN, Proepithelin, Progranulin -
基因ID
- 2896
-
UniProt
- P28799
抗原
-