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PD-1 抗体 (Extracellular Domain)

This anti-PD-1 antibody is a 山羊 多克隆 antibody detecting PD-1 in FACS, WB, IHC 和 ELISA. Suitable for 小鼠.
产品编号 ABIN325052
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for PD-1 抗体 (Extracellular Domain) (ABIN325052)

抗原

See all PD-1 (PDCD1) 抗体
PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

适用

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小鼠

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山羊

克隆类型

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多克隆

标记

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This PD-1 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA
  • 抗原表位

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    Extracellular Domain

    特异性

    Recognizes mouse PD-1 extracellular domain. Shows approximately 50 % cross-reactivity with rhPD-1.

    纯化方法

    Immunoaffinity purified

    免疫原

    Sf21-derived rmPD-1 extracellular domain.

    Type of Immunogen: Recombinant protein

    亚型

    IgG
  • 应用备注

    Approved: ELISA, Flo, IHC, WB

    Usage: Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse PD-1. The detection limit for rmPD-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody will detect PD-1 in cells and tissues. The working dilution is 2-15 μg/mL. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x106 with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse PD-1. The detection limit for rmPD-1 is approximately 0.03 ng/well.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    0.5 mL sterile PBS

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.4, 5 % trehalose

    注意事项

    Avoid frequent freeze thaws. Store undiluted.

    储存条件

    -20 °C

    储存方法

    Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

    别名

    PDCD1 / CD279 / PD-1

    背景

    Name/Gene ID: PDCD1
    Family: Apoptosis

    Synonyms: PDCD1, CD279, HPD-1, HPD-l, PD1, Protein PD-1, SLEB2, PD-1, CD279 antigen, Programmed cell death 1

    基因ID

    5133

    UniProt

    Q15116

    途径

    Cancer Immune Checkpoints
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