PD-1 抗体 (Extracellular Domain)
Quick Overview for PD-1 抗体 (Extracellular Domain) (ABIN325052)
抗原
See all PD-1 (PDCD1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Extracellular Domain
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特异性
- Recognizes mouse PD-1 extracellular domain. Shows approximately 50 % cross-reactivity with rhPD-1.
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纯化方法
- Immunoaffinity purified
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免疫原
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Sf21-derived rmPD-1 extracellular domain.
Type of Immunogen: Recombinant protein -
亚型
- IgG
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应用备注
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Approved: ELISA, Flo, IHC, WB
Usage: Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse PD-1. The detection limit for rmPD-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody will detect PD-1 in cells and tissues. The working dilution is 2-15 μg/mL. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x106 with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse PD-1. The detection limit for rmPD-1 is approximately 0.03 ng/well. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.5 mL sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.4, 5 % trehalose
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注意事项
- Avoid frequent freeze thaws. Store undiluted.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
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- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
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别名
- PDCD1 / CD279 / PD-1
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背景
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Name/Gene ID: PDCD1
Family: Apoptosis
Synonyms: PDCD1, CD279, HPD-1, HPD-l, PD1, Protein PD-1, SLEB2, PD-1, CD279 antigen, Programmed cell death 1 -
基因ID
- 5133
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UniProt
- Q15116
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途径
- Cancer Immune Checkpoints
抗原
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