Carboxypeptidase A2 抗体
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北京 101111
Quick Overview for Carboxypeptidase A2 抗体 (ABIN322590)
抗原
See all Carboxypeptidase A2 (CPA2) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes the pro form of human CPA2, but not the activated enzyme in Western blots. Species sequence homology: chimpanzee 99.5 % , canine 90 %, rat 87 %, mouse 86 % and chicken 70 %.
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纯化方法
- Protein G purified
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免疫原
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NS0-derived recombinant human rhCPA2.
Type of Immunogen: Recombinant protein -
亚型
- IgG2b
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anti-Carboxypeptidase A2 (Pancreatic) (CPA2) antibody
CPA2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3D8 unconjugated
anti-Carboxypeptidase A2 (Pancreatic) (CPA2) antibodyCPA2 适用: 人 WB, IHC, IHC (p), IF, FACS 宿主: 小鼠 Monoclonal 1C7 unconjugated
anti-Carboxypeptidase A2 (Pancreatic) (CPA2) (AA 117-206) antibodyCPA2 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 2E11 unconjugated
anti-Carboxypeptidase A2 (Pancreatic) (CPA2) antibodyCPA2 适用: 人 WB, IHC, IHC (p), IF, FACS 宿主: 小鼠 Monoclonal 1D8 unconjugated
anti-Carboxypeptidase A2 (Pancreatic) (CPA2) (AA 115-419) antibodyCPA2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A2 (Pancreatic) (CPA2) (AA 1-417) antibodyCPA2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A2 (Pancreatic) (CPA2) (AA 1-417) antibodyCPA2 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Carboxypeptidase A2 (Pancreatic) (CPA2) (AA 245-350) antibodyCPA2 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A2 (Pancreatic) (CPA2) antibodyCPA2 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A2 (Pancreatic) (CPA2) (AA 26-417) antibodyCPA2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), IP (25 μg/mL), Neut, WB (1 - 2 μg/mL)
Usage: Suitable for use in Western blot, Neutralization, Direct ELISA and Immunoprecipitation. Western blot:1-2 μg/mL the detection limit is ~10 ng/lane under non-reducing and reducing conditions. will increase sensitivity by 5 to 50 fold. Neutralization: preincubate with the activated enzyme at different molar ratios at room temperature for 30 min. Then assay with N-acetyl-Phe-Thiaphe-OH. 50 % of the enzymatic activity was inhibited at ~1.5 μg/mL (IC50) under conditions in which the enzyme was. present at 0.1 μg/mL and the substrate concentration was 0.1 mM. Considering the. molecular masses of the enzyme (42kD) and the antibody (150kD), IC50 was achieved at. approximately 5:1 Molar ratio of the antibody to the enzyme. Immunoprecipitation: 25 μg/mL. Direct ELISA: 0.5-1.0 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute, 1 mL sterile PBS.
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浓度
- Lot specific
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缓冲液
- Lyophilized powder from PBS, 5 % trehalose. Reconstitute, 1 mL sterile PBS.
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- Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))
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别名
- CPA2
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背景
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Name/Gene ID: CPA2
Subfamily: Metallopeptidase M14
Family: Exopeptidase
Synonyms: CPA2, Carboxypeptidase A2 -
基因ID
- 1358
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UniProt
- P48052
抗原
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