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GFP 抗体

Name: GFP 抗体
Brand: antibodies-online
SKU: ABIN3210020
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated

Western Blot showing using GFP antibody used against extracts from HCC827 cells, untransfected (1) and transfected with GFP (2).

FC analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP antibody .

FACS

Immunocytochemistry analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP antibody .

IHC

Western Blot showing GFP antibody used against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).

4 images

产品编号 ABIN3210020

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
175

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
宿主
74

73

35

11

9

6

2

1
小鼠
Compatible Secondaries
克隆类型
121

85
单克隆
标记
104

14

12

9

6

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
173

87

55

42

33

31

29

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

纯化方法

Purified

免疫原

GFP antibody was raised in Mouse using a purified recombinant fragment of GFP expressed in E. coli as the immunogen.

克隆位点

4B10B2

亚型

IgG2a

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应用备注

ELISA: 1:10,000, FC: 1:200-1:400, IHC: 1:200-1:1000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

Purified antibody supplied in PBS containing 0.03 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

储存条件

4 °C/-20 °C

储存方法

Store at 4 °C for short term storage. Store at -20 °C for long term storage.
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

物质类

Viral Protein

背景

Green Fluorescent Protein (GFP) tagging provide an excellent means for monitoring gene expression and protein localization in living cells, so that it is widely accepted by molecular and cell biological research. Monoclonal anti-GFP antibody can detect GFP and its variants on Western blotting, Immunoprecipitation and Immunocytochemistry. Synonyms: Monoclonal GFP antibody, Anti-GFP antibody, Green Fluorescent Protein antibody.