Isocitrate Dehydrogenase 抗体
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北京 101111
Quick Overview for Isocitrate Dehydrogenase 抗体 (ABIN3197487)
抗原
See all Isocitrate Dehydrogenase (IDH) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-IDH | Isocitrate dehydrogenase (Cellular [compartment marker] of mitochondrial matrix)
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交叉反应 （详细）
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Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Glycine max, Lycopersicum chilense, Nicotiana benthamiana, Oryza sativa, Solanum lycopersicum, Pisum sativum, Solanum sogarandium, Solanum tuberosum, Zea mays
Not reactive in: Chlamydomonas reinhardtii -
预测反应
- Brachypodium distachyon,Brassica napus,Capsella rubella,Citrus sinensis,Hordeum vulgare,Malus x domestica,Medicago truncatula,Nicotiana tabacum,Phaseolus vulgaris,Theobroma cacao,Triticum aestivum,Vitis vinifera,Zea mays
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated peptide 1 and peptide 2 conserved in all higher plants mitochondrial
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anti-Isocitrate Dehydrogenase (IDH) antibody
IDH 适用: 人, Pig, Vicia faba WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase (IDH) antibodyIDH 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase (IDH) (Middle Region) antibodyIDH 适用: 人 WB, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1:400 (IF), 1 : 5 000 (WB)
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说明
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cellular [compartment marker] of mitochondrial matrix
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).
: "Mitochondrial targeting of the Arabidopsis F1-ATPase γ-subunit via multiple compensatory and synergistic presequence motifs." in: The Plant cell, Vol. 24, Issue 12, pp. 5037-57, (2013) (PubMed).
: "Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism." in: Planta, Vol. 232, Issue 6, pp. 1371-82, (2010) (PubMed).
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- Isocitrate Dehydrogenase (IDH)
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背景
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Background: Plant NADH dependent isocitrate dehydrogenase enzyme is located in mitochondrial matrix. This enzyme is classified as an oxidoreductase and its function is to catalyze a reaction in the citric acid cycle, specifically the sequential dehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. It removes hydrogens from its substrate, isocitrate. In addition to this process, it functions as a decarboxylase, removing a CO2 from the six-carbon substrate to form a five-carbon product mentioned above as a-ketoglutarate. There are two forms of this enzyme NADP+ and NAD+ dependent.
Additional information: Peptide used to elicit this antibody is not conserved in NADPH dependent anzymes, partially conserved across eukaryotic Idh subunits, Some conservation across bacterial which contain the NAD-dependent form of Idh (as opposed to the NADP-dependent form)
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分子量
- 39 | 45 kDa (Arabidopsis thaliana)
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基因ID
- 816218
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UniProt
- P93032
抗原
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