Flow Cytometry: 20 μg/mL, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen sections: 1-10 μg/mL. Immunohistochemistry on Paraffin sections: 1-10 μg/mL. This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining eg. pronase or trypsin. Recommended Positive Control: Human Tonsil. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
Tris buffered saline containing 0.09 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
CD40
别名
CD40
背景
CD40 is a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centres, and is vital in T cell-B cell interactions.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5