CD34 抗体
Quick Overview for CD34 抗体 (ABIN319717)
抗原
See all CD34 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognises the Class II epitope on CD34. Ultrastructure studies, using an indirect immunogold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium, a slight reaction with an intracytoplasmic vesicular compartment is shown. Predominant staining is on the endothelial cell membrane. Some cross reactions with vascular associated adventitia and some basement membranes. Clone QBEND-10 immunoprecipitates a glycoprotein of Mr 110 kDa expressed on haemopoietic cells and on the established myeloid leukemic cell line KG1a.
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纯化方法
- Affinity Chromatography on Protein A
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免疫原
- Human endothelial cell membrane vesicles. Spleen cells from immunised NZB mice were fused with cells of the mouse NSO myeloma cell line.
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亚型
- IgG1
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anti-CD34 (CD34) (AA 366-382), (C-Term) antibody
CD34 适用: 人, 大鼠, 小鼠 FACS, WB, IHC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 201-300) antibodyCD34 适用: 人, 大鼠, 小鼠, 兔 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 301-385) antibodyCD34 适用: 人, 大鼠, 小鼠, 兔, 小鸡, 山羊 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 36-287) antibodyCD34 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (C-Term) antibodyCD34 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) antibodyCD34 适用: 人, 大鼠 FACS, IHC, StM 宿主: 兔 Monoclonal HPCA1-2598R unconjugated Recombinant Antibody
anti-CD34 (CD34) antibodyCD34 适用: 人, Non-Human Primate FACS, WB, IP, IHC (p), IHC (fro), Func 宿主: 小鼠 Monoclonal QBEnd-10 unconjugated
anti-CD34 (CD34) antibody (PE)CD34 适用: 人 FACS, ICC 宿主: 小鼠 Monoclonal 4H11[APG] PE
anti-CD34 (CD34) (AA 151-385) antibodyCD34 适用: 人, 大鼠 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 32-290) antibodyCD34 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/500-1/1000): This product does not require antigen retrieval using heat treatment or protein digestion. However staining of paraffin embedded formalin fixed tissues may be enhanced by enzyme pre-treatment using pronase or heat treatment using citrate buffer. Recommended positive control: Tonsil, Bone Marrow.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS containing 0.09 % Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD34
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别名
- CD34
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背景
- CD34 is a single chain transmembrane glycoprotein of 105 - 120kD. Expressed on haemopoietic cells, including bone marrow and blood. It is expressed on the majority of cells in acute lymphoid leukaemia (ALL) as well as cells in acute myelogenous leukaemia (AML). It is also found on capillary endothelial cells. There are three classes of CD34 epitopes defined by differential sensitivity to enzymatic cleavage with glycoprotease from Pasteurella haemolytica and with neuraminidase.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
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基因ID
- 947
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NCBI登录号
- NP_001020280
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UniProt
- P28906
抗原
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