Caspase 3 抗体
(1 reference)
(1 validation)Quick Overview for Caspase 3 抗体 (ABIN3188045)
抗原
See all Caspase 3 (CASP3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody detects endogenous Active Caspase-3 protein.
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产品特性
- Mouse Monoclonal to Active Caspase-3.
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纯化方法
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
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免疫原
- Recombinant Protein
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亚型
- IgG1
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anti-Caspase 3 (CASP3) (AA 60-100) antibody
CASP3 适用: 人, 小鼠, 大鼠, Cow, 山羊 WB, IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 67-175) antibodyCASP3 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 220-236), (C-Term) antibodyCASP3 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 11-120) antibodyCASP3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (cleaved) antibodyCASP3 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (Internal Region) antibodyCASP3 适用: 人, 小鼠, 大鼠, Cow WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (pSer150) antibodyCASP3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 29-175) antibodyCASP3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 29-175) antibodyCASP3 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Caspase 3 (CASP3) (AA 29-175) antibodyCASP3 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1000, IHC 1:100-200
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限制
- 仅限研究用
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- by
- Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #100061
- 日期
- 2016.08.31
- 抗原
- Caspase 3 (active)
- Lot Number
- Method validated
- Western Blotting
- Positive Control
- Mouse cerebellar tissue samples at post natal day P5
- Negative Control
- Notes
- The antibody recognized both the active and non-active form of Caspase3 and worked well in the experimental conditions tested. The product is therefore indicated for the WB use.
- Primary Antibody
- Secondary Antibody
- ABIN3179097
- Full Protocol
- Mouse cerebellar tissue samples at post natal day (P) 5 were lysed in 150 µL of lysis buffer.
- Protein concentration was determined using Bradford reagen (Sigma B6916) according to the manufacturer's recommendation.
- 30 and 60µg total protein were denatured in sample buffer for 5min at 95°C and then cooled on ice.
- Proteins were separated on a denaturing homemade 15% SDS-PAGE gel at a constant voltage of 180V for 70min.
- Proteins were transferred in a transfer buffer to nitrocellulose membrane (Amersham Biosciences, RPN203D, lot 18100/66/0207) at constant amperage of 400mA for 60min.
- Blocking of the membrane with blocking solution (0.01M PBS, 0.1% tween, 2% Bovine Serum Albumin) for 1h at RT.
- Incubation with the primary antibody ABIN3179097 diluted 1:1000 in blocking solution ON at 4°C.
- 3 washes in PBS-0.1% tween for 15min at RT.
- Incubation with the anti-mouse horseradish peroxidase (HRP) secondary antibody (Santa Cruz, SC2005, lot H0406) diluted 1:10000 in blocking solution for 1h at RT.
- Washes in PBS-0.1% tween for 15min at RT.
- Chemiluminescence detection using homemade ECL (1min incubation at RT) and revealed on HYPERFILM EGL (Amersham Biosciences, RPN2013K, lot 04450300115); exposure 30sec.
- Proteins were revealed using the ECL technique on western blot films for 30 seconds.
- Experimental Notes
- A band around 30-35 kDa and a band around 17kDa were detected with the experimental procedures previously described.
- A protein load of 30µg is recommended, as in the 60µg load, weak non-specific bands were detected around 15 and 30kDa.
生效 #100061 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![P5 mouse cerebellum, 30 seconds exposition. 60µg (left) and 30µg (right) protein loaded. The inactive procaspase 3 is visible at a MW of approximately 32kDa. The p17 subunit of active Casp3 is revealed between the 15kDa and 20kDa bands of the MW marker.]()
P5 mouse cerebellum, 30 seconds exposition. 60µg (left) and 30µg (right) protein loaded. The inactive procaspase 3 is visible at a MW of approximately 32kDa. The p17 subunit of active Casp3 is revealed between the 15kDa and 20kDa bands of the MW marker.
Full Methods -
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状态
- Liquid
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Store at -20°C.
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: "Alpha-1-Antitrypsin Protects Vascular Grafts of Brain-Dead Rats Against Ischemia/Reperfusion Injury." in: The Journal of surgical research, Vol. 283, pp. 953-964, (2023) (PubMed).
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: "Alpha-1-Antitrypsin Protects Vascular Grafts of Brain-Dead Rats Against Ischemia/Reperfusion Injury." in: The Journal of surgical research, Vol. 283, pp. 953-964, (2023) (PubMed).
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- Caspase 3 (CASP3)
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别名
- Caspase-3
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基因ID
- 836
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UniProt
- P42574
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
抗原
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