ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1,0 mg/mL
缓冲液
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Normal tissues are characterized by a balance between cellular stasis, cell proliferation, cell differentiation and cell death. Aberrant regulation of any of these cell processes can result in cancer. Cell death during embryogenesis, tissue atrophy and normal tissue turnover is called apoptosis and is characterized by cytoplasmic and nuclear condensation, nuclear disorganization and fragmentation of genomic DNA into 180-200 base pair oligomers. Five ionomycin-inducible complementary cDNAs, designated PAR1, 2, 3, 4 and 5, have been isolated from the prostate cancer cell line AT-3. Nucleotide sequencing identified PAR1 as the rat homolog of MKP-1, PAR2 as the injury-inducible gene HB-EGF, and PAR3 as the serum-induced gene Cyr61. PAR4 and PAR5 sequences were not found to correspond to any previously described proteins. PAR4 (prostate apoptosis response 4) is specifically expressed by cells entering apoptosis and is not induced during growth factor stimulation, oxidative stress, necrosis or growth arrest. The PAR4 gene encodes a protein with a putative nuclear localization signal and carboxy terminal leucine zipper.Synonyms: PRKC apoptosis WT1 regulator protein, Par-4, Prostate apoptosis response 4 protein