JIP3 抗体
Quick Overview for JIP3 抗体 (ABIN317721)
抗原
See all JIP3 (MAPK8IP3) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of JIP-3 protein.(region surrounding Gln649)
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交叉反应 （详细）
- Species reactivity (tested):Human and Mouse.
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纯化方法
- Affinity Chromatography using epitope-specific Immunogen.
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anti-Mitogen-Activated Protein Kinase 8 Interacting Protein 3 (MAPK8IP3) (Internal Region) antibody
MAPK8IP3 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 Interacting Protein 3 (MAPK8IP3) antibodyMAPK8IP3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 Interacting Protein 3 (MAPK8IP3) (Internal Region) antibodyMAPK8IP3 适用: 人, 小鼠 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 Interacting Protein 3 (MAPK8IP3) (Internal Region) antibodyMAPK8IP3 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 Interacting Protein 3 (MAPK8IP3) (Center) antibodyMAPK8IP3 适用: 人, 小鼠, 大鼠, Cow WB, IF, IHC, IC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 Interacting Protein 3 (MAPK8IP3) (AA 621-670) antibodyMAPK8IP3 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 Interacting Protein 3 (MAPK8IP3) (Internal Region) antibodyMAPK8IP3 适用: 人, 小鼠 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/20000approx. 1/40000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200. Immunofluorescence: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- JIP3 (MAPK8IP3) (Mitogen-Activated Protein Kinase 8 Interacting Protein 3 (MAPK8IP3))
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别名
- JIP-3
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背景
- C-Jun NH2-terminal kinases (JNKs) are distant members of the MAP kinase family. JNK1 is activated by dual phosphorylation at a Thr-Pro-Tyr motif in response to ultraviolet (UV) light, and it functions to phosphorylate c-Jun at amino terminal serine regulatory sites Ser 63 and Ser 73, resulting in transcriptional activation. Two additional JNK family members, JNK2 and JNK3, have been identified. JIP-1 (for JNK interacting protein-1) has been identified as a cytoplasmic inhibitor of JNK that retains JNK in the cytoplasm, thereby inhibiting JNK-regulated gene expression. Evidence suggests that JNK1 and JNK2 bind to JIP-1 with greater affinity than to ATF-2 and c-Jun, which are targets of the JNK signaling pathway. JIP-1 contains an amino terminal JNK binding domain and a carboxy terminal SH3 domain. ATF-2 and c-Jun also contain the JNK binding domain and are thought to compete with JIP-1 for JNK binding. Multiple splice variants of JIP-1, including JIP-1b, JIP-1c (also designated islet-brain 1 or IB-1), JIP-2a, JIP-2b and JIP-3, have been identified in brain.Synonyms: C-jun-amino-terminal kinase-interacting protein 3, JIP3, JNK MAP kinase scaffold protein 3, JNK-interacting protein 3, MAPK8IP3, Mitogen-activated protein kinase 8-interacting protein 3
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基因ID
- 23162
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NCBI登录号
- NP_001035529
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UniProt
- Q9UPT6
抗原
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