Interferon gamma 抗体
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- 抗原 See all Interferon gamma (IFNG) 抗体
- Interferon gamma (IFNG)
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适用
- Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Interferon gamma antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody recognises both native and recombinant IFN gamma.
- 产品特性
- Synonyms: IFN-gamma, gamma IFN
- 纯化方法
- Purified IgG prepared by affinity chromatography on Protein G
- 克隆位点
- 7B6
- 亚型
- IgG1
- Top Product
- Discover our top product IFNG Primary Antibody
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- 应用备注
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Western blot. Flow cytometry: 1/500 - 1/2000, use 10 μL of the suggested working dilution to label 1x10^6cells in 100 μL (Membrane permeabilisation is required for this application, we recommendthe use of Leukoperm). Immunohistochemistry on paraffin sections (requires antigen retrieval using heattreatment prior to staining, Sodium citrate buffer pH 6.0 is recommended). Does not work in ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline pH 7.4, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- Interferon gamma (IFNG)
- 别名
- IFNG / Interferon gamma (IFNG 产品)
- 背景
- Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.Synonyms: IFN-gamma, gamma IFN
- 基因ID
- 281237
- UniProt
- P07353
- 途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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