Hexokinase 1 抗体 (N-Term)
Quick Overview for Hexokinase 1 抗体 (N-Term) (ABIN317095)
抗原
See all Hexokinase 1 (HK1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-917, N-Term
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特异性
- The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein.
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交叉反应 （详细）
- Species reactivity (tested):Human, mouse (see pictures below).
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产品特性
- Synonyms: HK1, Hexokinase type I, Brain form hexokinase
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纯化方法
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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免疫原
- Recombinant Human Hexokinase 1 (1-917 aa) purified from E. coli
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亚型
- IgG2a
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anti-Hexokinase 1 (HK1) (AA 413-540) antibody
HK1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hexokinase 1 (HK1) antibodyKO Validated HK1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Monoclonal unconjugated
anti-Hexokinase 1 (HK1) (AA 20-300) antibodyHK1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Hexokinase 1 (HK1) (AA 1-916) antibodyHK1 适用: 人 WB, IP, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Hexokinase 1 (HK1) antibodyHK1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 3A10 unconjugated
anti-Hexokinase 1 (HK1) (AA 78-108), (N-Term) antibodyHK1 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB3921-RB3922 unconjugated
anti-Hexokinase 1 (HK1) antibodyHK1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7A7 unconjugated
anti-Hexokinase 1 (HK1) antibodyHK1 适用: 人 WB, ICC, IF, IHC (p), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Hexokinase 1 (HK1) (AA 80-215) antibodyHK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hexokinase 1 (HK1) (AA 1-916) antibodyHK1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA. Western blot (1: 1,000). Immunofluorescence (1: 1,000). Immunohistochemistry on Paraffin Sections (5 μg/mL): This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Hexokinase 1 (HK1)
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别名
- Hexokinase-1
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背景
- Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia.Synonyms: Brain form hexokinase, HK1, Hexokinase type I
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基因ID
- 3098
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UniProt
- P19367
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途径
- Carbohydrate Homeostasis, Warburg Effect
抗原
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