This antibody recognises FOXP1. The antibody produced by clone JC12 does not recognise closely related molecules FOXP2, FOXP3 or FOXP4. Cross reacts with Mouse.
产品特性
Synonyms: Forkhead box protein P1, HSPC215
纯化方法
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Western Blot: Detects a band of approximately 85 kDa in Tonsil extracts. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200-1/500. This product requires antigenretrieval using heat treatment prior to staining of paraffin sections, 1 mM EDTA pH 8.0 isrecommended for this purpose. Histology Positive Control Tissue: Human tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
FOXP1 is an 85kD member of the winged helix/forkhead transcription factor family. The FOXP1 transcription factor is widely expressed in human tissues and is predominantly localised to the nucleus. However, the level of expression does vary between cells and between different tissues. The expression of FOXP1 is reported to be abnormal in a range of solid tumours. Studies suggest that FOXP1 functions as a transcriptional repressor.Synonyms: Forkhead box protein P1, HSPC215