Clusterin 抗体
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北京 101111
Quick Overview for Clusterin 抗体 (ABIN307177)
抗原
See all Clusterin (CLU) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human clusterin, alpha at 40kD, and precursor protein.
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纯化方法
- Protein G purified
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免疫原
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Native clusterin purified from human serum.
Type of Immunogen: Purified protein -
亚型
- IgG1 kappa
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL. Detects human clusterin a chain (40kD) in RIPA lysates from HeLa cells, human serum and MCF-7 cell lysates. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 40 %
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Aliquot to Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.
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- Clusterin (CLU)
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别名
- CLU / Clusterin
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背景
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Name/Gene ID: CLU
Synonyms: CLU, APO-J, Apolipoprotein J, CLU1, Clusterin, Complement lysis inhibitor, CLI, AAG4, Aging-associated protein 4, Ku70-binding protein 1, KUB1, SGP2, APOJ, SGP-2, Sulfated glycoprotein 2, CLU2, Complement cytolysis inhibitor, NA1/NA2, SP-40 -
基因ID
- 1191
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UniProt
- P10909
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途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
抗原
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