SIAH1 抗体
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北京 101111
Quick Overview for SIAH1 抗体 (ABIN307027)
抗原
See all SIAH1 抗体适用
宿主
克隆类型
标记
应用范围
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交叉反应
- 人
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产品特性
- SIAH1 (seven in absentia homolog 1), E3 Ubiquitin Ligase,Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1. Both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death, 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS), 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it,3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity.
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纯化方法
- Ammonium Sulfate Precipitation
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免疫原
- Synthetic peptide derived from the human SIAH-1 protein.
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亚型
- IgG
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anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibody
Verified SIAH1 适用: 人, 大鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-313) antibodySIAH1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-110) antibodySIAH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C5 unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibodySIAH1 适用: 人, 大鼠, 小鼠, 犬, 斑马鱼, Cow, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibodySIAH1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 191-240) antibodySIAH1 适用: 人, 大鼠, 小鼠, 犬, 斑马鱼, Cow, 豚鼠, 马, 兔, 非洲爪蟾, 小鸡, Bat, Hamster, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (C-Term) antibodySIAH1 适用: 人, 大鼠, 小鼠, 犬, 斑马鱼, Cow, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 201-282) antibodySIAH1 适用: 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-313) antibodySIAH1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibody (Biotin)Verified SIAH1 适用: 人, 大鼠, 小鼠 WB, ELISA, IP 宿主: 山羊 Polyclonal Biotin
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应用备注
- Antibody can be used for Western blotting (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.
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限制
- 仅限研究用
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缓冲液
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
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别名
- SIAH1
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UniProt
- Q8IUQ4
抗原
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