BrdU 抗体 (FITC)
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北京 101111
Quick Overview for BrdU 抗体 (FITC) (ABIN306900)
抗原
See all BrdU 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. The FITC conjugate is particularly useful in these applications as a secondary antibody is not necessary for visualization. Also, a specific anti-FITC antibody conjugate can be used for signal amplification if desired.
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纯化方法
- Protein A/G Chromatography
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免疫原
- Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin was used to immunize BALB/c mice
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亚型
- IgG1
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anti-Bromodeoxyuridine (BrdU) antibody
BrdU FACS, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal Bu20a unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), WB 宿主: 小鼠 Monoclonal A7 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, FACS, IF, WB, ELISA, FM, DB 宿主: 小鼠 Monoclonal 29G6-E8 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 小鼠 Monoclonal BRD469 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), ICC, IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 小鼠 Monoclonal 4B6C12 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 兔 Monoclonal BRD2888R unconjugated Recombinant Antibody
anti-Bromodeoxyuridine (BrdU) antibody (PE)BrdU ICFC 宿主: 小鼠 Monoclonal Bu20a PE
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应用备注
- Reacts with cells containing incorporated BrdU, showing a clear, nucleus confined, speckled pattern. Use at 1-5 μg/106 cells for flow cytometry. Optimal concentration should be evaluated by serial dilutions.
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限制
- 仅限研究用
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缓冲液
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- BrdU (Bromodeoxyuridine (BrdU))
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别名
- BrdU (bromodeoxyuridine)
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物质类
- Chemical
抗原
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