BUB1B
(Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))
抗原表位
AA 883-1052
适用
小鼠
宿主
绵羊
克隆类型
多克隆
标记
This BUB1B antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (IHC)
交叉反应
小鼠
产品特性
Mitotic checkpoint kinase MAD3L,Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.
纯化方法
Ammonium Sulfate Precipitation
免疫原
Recombinant protein corresponding to amino acids 883-1052 of the mouse Bub1b protein.
BUB1B
适用: 人, 小鼠
WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
Antibody detects Bub1b in mitotic cells seperated from NIH/3T3 cells by IP/Westen blot (1-10 μg/mL) analysis. Detects a 130 kDa band. Optimal concentration should be evaluated by serial dilutions.
限制
仅限研究用
缓冲液
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
抗原
BUB1B
(Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))