NAPA 抗体
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北京 101111
Quick Overview for NAPA 抗体 (ABIN306712)
抗原
See all NAPA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Human alpha -SNAP
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交叉反应
- Cow, 人, 大鼠
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产品特性
- alpha/beta SNAP, mouse anti alpha soluble NSF attachment protein,SNAPA,SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. Beta-SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas alpha-SNAP is expressed in all mammalian tissues, beta-SNAP is expressed only in brain.
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纯化方法
- The antibody was purified to >95% homogeneity by standard chromatographic techniques
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免疫原
- Balb/C mice were immunized with recombinant human alpha -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by Western blot.,Monoclonal antibody 15D4 obtained immunization of Balb/C mice with recombinant human alpha -SNAP
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亚型
- IgG1
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anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (Center) antibody
NAPA 适用: 人, 小鼠, 大鼠 WB, ICC, IF, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) antibodyNAPA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) antibodyNAPA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) antibodyNAPA 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 78-295) antibodyNAPA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) antibodyNAPA 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) antibody (APC)NAPA 适用: 人 IHC, ICC, IF, FACS 宿主: 兔 Polyclonal APC
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应用备注
- Optimal concentration should be evaluated by serial dilutions. Clone 4E4 anti-?SNAP specifically recognizes ?-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and does not recognize ?-SNAP Application: A dilution of 0.5-5 μg/mL is suggested for western blot and 2-10 μg/mL for immunoprecipitation.
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限制
- 仅限研究用
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缓冲液
- 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol. No preservatives have been included in the antibody solution.,100 μg monoclonal antibody in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol. No preservatives have been added.
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
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别名
- alpha SNAP
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途径
- Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
抗原
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