NAPA 抗体 (Center)
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- 抗原 See all NAPA 抗体
- NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
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抗原表位
- Center
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NAPA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 猕猴, Chimpanzee
- 交叉反应 （详细）
- Rhesus Monkey (100 %), Chimpanzee (100 %)
- 产品特性
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Rabbit Polyclonal antibody to alpha SNAP (N-ethylmaleimide-sensitive factor attachment protein, alpha)
alpha SNAP antibody [N2C3] - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human alpha SNAP. The exact sequence is proprietary.
- 亚型
- IgG
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anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) antibody
NAPA 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) antibodyNAPA 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 78-295) antibodyNAPA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) antibodyNAPA 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) antibody (APC)NAPA 适用: 人 IHC, ICC, IF, FACS 宿主: 兔 Polyclonal APC
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- 应用备注
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation Assay-dependent dilution Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* ImmunoprecipitationAssay-dependent dilution Western blot1:1000-1:10000*
- 说明
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Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- 抗原
- NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
- 别名
- alpha SNAP (NAPA 产品)
- 别名
- SNAPA antibody, wu:fa26c04 antibody, zgc:63906 antibody, napb antibody, 1500039N14Rik antibody, AW209189 antibody, RA81 antibody, SNARE antibody, a-SNAP antibody, hyh antibody, alpha-SNAP antibody, Alpha-SNAP antibody, NSF attachment protein alpha antibody, N-ethylmaleimide-sensitive factor attachment protein, alpha b antibody, NSF attachment protein alpha L homeolog antibody, N-ethylmaleimide-sensitive factor attachment protein, alpha antibody, N-ethylmaleimide sensitive fusion protein attachment protein alpha antibody, soluble NSF attachment protein antibody, NAPA antibody, napab antibody, napa.L antibody, napa antibody, EHI_000300 antibody, Napa antibody, LOC102577508 antibody
- 背景
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The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37 % , 60 %, and 67 % identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types. Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different.
Cellular Localization: Membrane, Peripheral membrane protein - 分子量
- 33 kDa
- 基因ID
- 8775
- 途径
- Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
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