PTP PEST, protein tyrosine phosphatase PEST, Tyrosine-protein phosphatase non-receptor type 12, Protein-tyrosine phosphatase G1,PP2A is a heterotrimeric phosphatase, ccomposed of A, B and C subunits. The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. Three families of B subunits have been identified thus far. The prototypical B (or PR55) family of subunits is encoded by three genes, B , B and B . The B' (or B56) family consists of at least seven splice variants derived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals. B is a novel isoform of the B class of PP2A regulatory subunits. It is widely expressed in rat tissues and appears to be involved in targeting PP2A to the cytosolic compartment.
纯化方法
Ammonium Sulfate Precipitation
免疫原
Synthetic peptide conjugated to KLH derived from the N-terminus of PP2A/B delta
Detects PP2A/B delta protein in rat testis by Western blot. Detects a 53 kDa band in rat brain, heart, lung, placenta, skeletal muscle, spleen, testis and thymus tissues lysates. Optimal concentration should be evaluated by serial dilutions. Applications: Western Blotting, Immunofluorescence
限制
仅限研究用
缓冲液
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles