P110 (Mitochondrial Membrane Protein) 抗体
The 小鼠 单克隆 anti-P110 (Mitochondrial Membrane Protein) antibody (Clone 2G2) (ABIN306552) specifically detects P110 (Mitochondrial Membrane Protein) in IF 和 WB.
The antibody is reactive with 人 和 猴 samples.
产品编号 ABIN306552
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for P110 (Mitochondrial Membrane Protein) 抗体 (ABIN306552)
抗原
P110 (Mitochondrial Membrane Protein)
适用
人, 猴
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunofluorescence (IF), Western Blotting (WB)
克隆位点
2G2
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原理
- Mouse anti Human P110 (mitochondrial membrane protein)
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交叉反应
- 人
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产品特性
- P110 is a human mitochondrial protein with a molecular weight of 110 kDa. Close examination of the staining pattern in HeLa and Fanconi's Anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. The epitope targeted may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells. The mouse monoclonal P110 antibody labels mitochondria in human cells, as assessed by double staining with either Rhodamine 123 or a polyclonal antibody to mitochondrial matrix HSP-60 proteins. The P110 antigen has an approximate isoelectric point of 6.5 that copartitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. The P110 staining pattern in HeLa and Fanconi's anaemia cells reveals differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi's anaemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules. After stabilization with aldehyde- or alcohol-based fixation protocols that optimize the preservation of cytoskeletal components, the epitope targeted by the 2G2 antibody may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells.
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纯化方法
- Protein A/G Chromatography
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免疫原
- Skeletal fracrtion of HeLa-S3 cells
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亚型
- IgG1
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应用备注
- Application: Western Blot at 2-10 μg/mL. Immunofluorescence staining.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- phosphate buffered saline with 0.08 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- P110 (Mitochondrial Membrane Protein)
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背景
- 2G2, mitochondrial membrane protein, p110
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UniProt
- Q9KGY0
抗原
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