P110 (Mitochondrial Membrane Protein) 抗体
The 小鼠 单克隆 anti-P110 (Mitochondrial Membrane Protein) antibody is suitable to detect P110 (Mitochondrial Membrane Protein) in samples from 人 和 猴. It has been validated for IF 和 WB.
产品编号 ABIN187409
发货至:
中国
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Quick Overview for P110 (Mitochondrial Membrane Protein) 抗体 (ABIN187409)
抗原
P110 (Mitochondrial Membrane Protein)
适用
人, 猴
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunofluorescence (IF), Western Blotting (WB)
克隆位点
2G2
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特异性
- Reacts with a mitochondrial membrane protein present in humans and monkeys. Species Reactivity: Human and monkey. Does not cross-react with mouse, rat, or chicken.
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免疫原
- Skeletal fraction of HeLa-S3 cells.
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亚型
- IgG1
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应用备注
- Immunofluorescence Western blot: Use at 2-10 µg/mL. The optimal dilution for a specific application should be determined by the researcher.
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限制
- 仅限研究用
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缓冲液
- 100 µg purified IgG at 1 mg/mL in PBS with 0.08% sodium azide as a preservative. Purified from ascites fluid and sterilized by 0.2 µM filtration.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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- P110 (Mitochondrial Membrane Protein)
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背景
- P110 is a human mitochondrial protein with a molecular weight of 110 kDa. The epitope targeted may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells. The mouse monoclonal P110 antibody labels mitochondria in human cells, as assessed by double staining with either Rhodamine 123 or a polyclonal antibody to mitochondrial matrix HSP-60 proteins. The P110 antigen has an approximate isoelectric point of 6.5 that co-partitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. The P110 staining pattern in HeLa and Fanconi's anemia cells reveals differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi's anemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules. After stabilization with aldehyde- or alcohol-based fixation protocols that optimize the preservation of cytoskeletal components, the epitope targeted by the 2G2 antibody may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells.
抗原
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