Occludin 抗体 (C-Term)
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- 抗原 See all Occludin (OCLN) 抗体
- Occludin (OCLN)
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抗原表位
- C-Term
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适用
- 人, 小鼠, 大鼠, 犬
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Occludin antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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A GST fusion protein consisting of the C-terminal 150 amino acids of human occludin fused to GST.
Type of Immunogen: Fusion protein - 亚型
- IgG
- Top Product
- Discover our top product OCLN Primary Antibody
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- 应用备注
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Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-P (2.5 μg/mL), IP, WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- Occludin (OCLN)
- 别名
- OCLN / Occludin (OCLN 产品)
- 别名
- AI503564 antibody, Ocl antibody, ocln antibody, oclnb antibody, tpmt antibody, OCLN antibody, wu:fd23h10 antibody, wu:fi13c01 antibody, zgc:113992 antibody, zgc:56359 antibody, BLCPMG antibody, occludin antibody, occludin S homeolog antibody, occludin a antibody, Ocln antibody, OCLN antibody, ocln.S antibody, ocln antibody, oclna antibody
- 背景
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Name/Gene ID: OCLN
Synonyms: OCLN, Occludin, BLCPMG - 基因ID
- 100506658
- UniProt
- Q16625
- 途径
- Cell-Cell Junction Organization, Hepatitis C
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