Occludin 抗体 (C-Term)
Quick Overview for Occludin 抗体 (C-Term) (ABIN305888)
抗原
See all Occludin (OCLN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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品牌
- IHC-plus™
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特异性
- Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.
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纯化方法
- Immunoaffinity purified
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免疫原
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A GST fusion protein consisting of the C-terminal 150 amino acids of human occludin fused to GST.
Type of Immunogen: Fusion protein -
亚型
- IgG
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anti-Occludin (OCLN) (AA 431-522) antibody
OCLN 适用: 人, 小鼠, Pig WB, ELISA, FACS, IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 263-522) antibodyOCLN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (C-Term) antibodyOCLN 适用: 人 WB, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 423-522) antibodyOCLN 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-Occludin (OCLN) (AA 1-522) antibodyOCLN 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 1-522) antibodyOCLN 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 282-415) antibodyOCLN 适用: 人 WB, IHC (f) 宿主: 小鼠 Monoclonal OCLN-2181 unconjugated
anti-Occludin (OCLN) (AA 285-430) antibodyOCLN 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (C-Term) antibodyOCLN 适用: 人, 小鼠, 大鼠, 犬 WB, IHC, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal OC-3F10 unconjugated
anti-Occludin (OCLN) (AA 282-415) antibodyOCLN 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-415M unconjugated
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应用备注
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Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-P (2.5 μg/mL), IP, WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Occludin (OCLN)
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别名
- OCLN / Occludin
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背景
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Name/Gene ID: OCLN
Synonyms: OCLN, Occludin, BLCPMG -
基因ID
- 100506658
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UniProt
- Q16625
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途径
- Cell-Cell Junction Organization, Hepatitis C
抗原
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