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TJP2 抗体 (C-Term)

This anti-TJP2 antibody is a 兔 多克隆 antibody detecting TJP2 in WB, IHC, IF 和 IHC (p). Suitable for 人, 小鼠, 大鼠 和 犬.
产品编号 ABIN305886
发货至: 中国

Quick Overview for TJP2 抗体 (C-Term) (ABIN305886)

抗原

See all TJP2 抗体
TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

适用

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人, 小鼠, 大鼠, 犬

宿主

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克隆类型

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多克隆

标记

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This TJP2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    C-Term

    品牌

    IHC-plus™

    特异性

    Recognizes the ~130-160kD human ZO-2 protein, isoforms A or C. A band of unknown origin lower than 75kD may be observed in certain lysates.

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide derived from the C-terminal region of human or canine ZO-2.

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: IF (2.5 μg/mL), IHC, IHC-P (5 - 10 μg/mL), WB (1 - 3 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunofluorescence: 2.5 μg/mL. Cells need to be fixed with ice-cold ethanol for 30 minutes on ice prior to assays. Positive control: Western Blot: CaCO2 human colorectal adenocarcinoma, MDCK II canine kidney, KNRK rat kidney, and TMCK-1 mouse kidney cell lysates.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Aliquot to Avoid repeated freezing and thawing

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C, Long term: aliquot to Avoid freeze-thaw cycles, and store at -20°C.
  • 抗原

    TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

    别名

    TJP2 / ZO2 / ZO-2

    背景

    Name/Gene ID: TJP2
    Family: Occludin

    Synonyms: TJP2, C9DUPq21.11, DUP9q21.11, DFNA51, ZO-2, Zona occludens 2, Zonula occludens protein 2, Tight junction protein 2, Tight junction protein ZO-2, ZO2, X104, Zona occludens protein 2

    基因ID

    9414

    UniProt

    Q9UDY2
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