HLA-B27 抗体 (FITC)
This anti-HLA-B27 antibody is a 小鼠 单克隆 antibody detecting HLA-B27 in FACS. Suitable for 人.
Quick Overview for HLA-B27 抗体 (FITC) (ABIN304843)
抗原
See all HLA-B27 抗体
HLA-B27
(HLA Class I Histocompatibility Antigen, B-27 alpha Chain (HLA-B27))
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This HLA-B27 antibody is conjugated to FITC
应用范围
Flow Cytometry (FACS)
克隆位点
HLA-ABC-m3
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特异性
- Recognizes the HLA-B27 alloantigen. Reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin. Affinity studies by Scatchard analysis showed that this has a higher affinity for HLA-B27 (9.7 x 108 M-1) than for HLA B7 (9.5 x 107 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.
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纯化方法
- Ion exchange chromatography
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免疫原
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Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
Type of Immunogen: Cells -
亚型
- IgG2a
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应用备注
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Approved: Flo (1:1)
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL of whole blood. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.09 % sodium azide, 0.2 % BSA.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- 4°C, avoid freezing.
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- HLA-B27 (HLA Class I Histocompatibility Antigen, B-27 alpha Chain (HLA-B27))
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别名
- HLA-B27
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途径
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原
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