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Ki-67 抗体 (AA 2860-3256)

Name: Ki-67 抗体 (AA 2860-3256)
SKU: ABIN3044570
Availability: OutOfStock

MKI67 适用: 人 IHC, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

Anti-Ki67 Picoband antibody, IHC(P): Human Tonsil Tissue (Ki-67 抗体 (AA 2860-3256))

IHC

Anti-Ki67 Picoband antibody, All lanes: Anti-Ki67 at 0. (Ki-67 抗体 (AA 2860-3256))

2 images

(46 references)

产品编号 ABIN3044570

发货至: 中国

Datasheet as PDF

抗原 See all Ki-67 (MKI67) 抗体

Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))
抗原表位
16

12

7

6

6

5

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2860-3256
适用
116

34

24

5

3

2

1

1
人
宿主
82

37

4

3
兔
Compatible Secondaries
克隆类型
71

54
多克隆
标记
80

8

8

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This Ki-67 antibody is un-conjugated
应用范围
63

56

39

36

27

26

23

16

13

10

5

5

5

3

2

2

1

1

1

1
Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

原理

Anti-Ki67/MKI67 Antibody Picoband®

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-Ki67/MKI67 Antibody Picoband® (ABIN3044570). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

E.coli-derived human Ki67 recombinant protein (Position: K2860-I3256).

亚型

IgG

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anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 2293-2478) antibody
MKI67 适用: 人 IHC, WB, FACS, IF, StM 宿主: 小鼠 Monoclonal MKI67-2461 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 2293-2478) antibody
MKI67 适用: 人 IHC, WB, FACS, IF, StM 宿主: 小鼠 Monoclonal MKI67-2462 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1160-1493) antibody
MKI67 适用: 人 IHC, WB, IHC (p) 宿主: 大鼠 Monoclonal 9C3 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 2815-2928) antibody
MKI67 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1160-1493) antibody
MKI67 适用: 人 IHC, WB, ELISA, FACS 宿主: 小鼠 Monoclonal 7B12B1 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody
MKI67 适用: 人, Cow IHC, WB, FACS, ICC 宿主: 小鼠 Polyclonal unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 2924-3256) antibody
MKI67 适用: 人 IHC, IHC (p) 宿主: 小鼠 Monoclonal 8H5 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 1160-1493) antibody
MKI67 适用: 人 IHC, IHC (p) 宿主: 大鼠 Monoclonal 5B10 unconjugated

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (AA 3088-3235) antibody
MKI67 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunofluorescence, 5 μg/mL, Human
1. Fonatsch, C., Duchrow, M., Rieder, H., Schluter, C., Gerdes, J. Assignment of the human Ki-67 gene (MKI67) to 10q25-qter. Genomics 11: 476-477, 1991. 2. Schonk, D. M., Kuijpers, H. J. H., vanDrunen, E., vanDalen, C. H., Geurts van Kessel, A. H. M., Verheijen, R., Ramaekers, F. C. S. Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10. Hum. Genet. 83: 297-299, 1989. 3. Traut, W., Scholzen, T., Winking, H., Kubbutat, M. H. G., Gerdes, J. Assignment of the murine Ki-67 gene (Mki67) to chromosome band 7F3-F5 by in situ hybridization. Cytogenet. Cell Genet. 83: 12-13, 1998.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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Chen, Li, Peng, Jie, Dongye, Cai, Feng, Li, Zeng, Lun, Chen, Xu: "The induction of autophagy against mitochondria-mediated apoptosis in lung cancer cells by a ruthenium (II) imidazole complex." in: Oncotarget, Vol. 7, Issue 49, pp. 80716-80734, (2018) (PubMed).

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Sun, Zhang, Wang, Shang, Zhou, Wang, Duan, Ruan, Gao, Dai, Qu, Liu, Ding, Wang, Wang, Dou: "MicroRNA-150 suppresses cell proliferation and metastasis in hepatocellular carcinoma by inhibiting the GAB1-ERK axis." in: Oncotarget, Vol. 7, Issue 10, pp. 11595-608, (2017) (PubMed).

Zhou, Wang, Xu, Wan, Qu, Fan, Chen, Sun, Liu: "Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo." in: Oncotarget, Vol. 7, Issue 14, pp. 18620-30, (2017) (PubMed).

Jiang, He, Wang, Zhang, Zhang, Yan, Wang, Che, Ke, Chen, Tong, Zhang, Wang, Li, Wan: "Mutant p53 induces EZH2 expression and promotes epithelial-mesenchymal transition by disrupting p68-Drosha complex assembly and attenuating miR-26a processing." in: Oncotarget, Vol. 6, Issue 42, pp. 44660-74, (2016) (PubMed).

Gao, Shen, He, Xiao: "GNRs@SiO₂-FA in combination with radiotherapy induces the apoptosis of HepG2 cells by modulating the expression of apoptosis-related proteins." in: International journal of molecular medicine, Vol. 36, Issue 5, pp. 1282-90, (2016) (PubMed).

Han, Wu, Wu, Huang, Cheng, Li, Sun, Xie, Zhou, Du: "Tumor-suppressive function of long noncoding RNA MALAT1 in glioma cells by downregulation of MMP2 and inactivation of ERK/MAPK signaling." in: Cell death & disease, Vol. 7, pp. e2123, (2016) (PubMed).

Zeng, Zhao, Ouyang, Zhao, Zhang, Chen, Yu, Lei: "Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium." in: Oncology letters, Vol. 12, Issue 2, pp. 815-824, (2016) (PubMed).

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Alsaegh, Miyashita, Zhu: "Expression of human papillomavirus is correlated with Ki-67 and COX-2 expressions in keratocystic odontogenic tumor." in: Pathology oncology research : POR, Vol. 21, Issue 1, pp. 65-71, (2015) (PubMed).

Li, Wang, Wu, Yang, Zhao, Chen, Xie, Li, Wei, Huang, Zhou, Du: "miR-494-3p Regulates Cellular Proliferation, Invasion, Migration, and Apoptosis by PTEN/AKT Signaling in Human Glioblastoma Cells." in: Cellular and molecular neurobiology, Vol. 35, Issue 5, pp. 679-87, (2015) (PubMed).

Liu, Wang, Wang: "Controllable organization and high throughput production of recoverable 3D tumors using pneumatic microfluidics." in: Lab on a chip, Vol. 15, Issue 4, pp. 1195-204, (2015) (PubMed).

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Cheng, Wang, Li, Wu, Han, Li, Chen, Xie, Huang, Du, Zhou: "MicroRNA-184 inhibits cell proliferation and invasion, and specifically targets TNFAIP2 in Glioma." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 27, (2015) (PubMed).

Xie, Zhu, Yang, Huang, Wu, Wang, Luo, Wang, Shao: "Solamargine triggers hepatoma cell death through apoptosis." in: Oncology letters, Vol. 10, Issue 1, pp. 168-174, (2015) (PubMed).

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抗原

Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

别名

MKI67 (MKI67 产品)

背景

Synonyms: Antigen KI-67,MKI67,
Tissue Specificity: Released primarily from activated T lymphocytes.
Background: Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
Sequence Similarities: Contains 1 FHA domain.

分子量

358 kDa

基因ID

4288

UniProt

P46013

途径

Glycosaminoglycan Metabolic Process