NONO 抗体 (N-Term)
Quick Overview for NONO 抗体 (N-Term) (ABIN3044530)
抗原
See all NONO 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-35, N-Term
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原理
- Anti-nmt55/p54nrb/NONO Antibody Picoband®
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序列
- MQSNKTFNLE KQNHTPRKHH QHHHQQQHHQ QQQQQ
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-nmt55/p54nrb/NONO Antibody Picoband® (ABIN3044530). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human nmt55/p54nrb, identical to the related mouse and rat sequences.
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亚型
- IgG
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anti-Non-POU Domain Containing, Octamer-Binding (NONO) (AA 184-385) antibody
NONO 适用: 人, 小鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3F8 unconjugated
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (AA 184-385) antibodyNONO 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2E7 unconjugated
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (AA 1-300) antibodyNONO 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (C-Term) antibodyNONO 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 豚鼠, 兔 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Non-POU Domain Containing, Octamer-Binding (NONO) antibodyNONO 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Monoclonal 8C11 unconjugated Recombinant Antibody
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (N-Term) antibodyNONO 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (C-Term) antibodyNONO 适用: 人, 犬, 猴, 马, Cow, 豚鼠, 兔, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (C-Term) antibodyNONO 适用: 人, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (N-Term) antibodyNONO 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 豚鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (AA 1-300) antibodyNONO 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. "Entrez Gene: NONO Non-POU domain containing, octamer-binding". 2. Dong B, Horowitz DS, Kobayashi R, Krainer AR (Oct 1993). "Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6". Nucleic Acids Res. 21 (17): 4085-92. 3. Traish AM, Huang YH, Ashba J, Pronovost M, Pavao M, McAneny DB, Moreland RB (Dec 1997). "Loss of expression of a 55 kDa nuclear protein (nmt55) in estrogen receptor-negative human breast cancer". Diagn. Mol. Pathol. 6(4): 209-21. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
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别名
- NONO
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背景
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Synonyms: Non-POU domain-containing octamer-binding protein,NonO protein,54 kDa nuclear RNA- and DNA-binding protein,55 kDa nuclear protein,DNA-binding p52/p100 complex, 52 kDa subunit,NMT55,p54 (nrb),p54nrb,NONO,NRB54,
Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines. .
Background: Non-POU domain-containing octamer-binding protein?is a?protein?that in humans is encoded by the?NONO gene. This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.?
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分子量
- 60 kDa
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基因ID
- 4841
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UniProt
- Q15233
抗原
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