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NONO 抗体 (N-Term)

NONO 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3044530
发货至: 中国
  • 抗原 See all NONO 抗体
    NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
    抗原表位
    • 15
    • 8
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-35, N-Term
    适用
    • 75
    • 35
    • 31
    • 15
    • 12
    • 6
    • 5
    • 5
    • 5
    • 3
    • 3
    • 1
    人, 小鼠, 大鼠
    宿主
    • 68
    • 6
    • 2
    克隆类型
    • 60
    • 16
    多克隆
    标记
    • 41
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NONO antibody is un-conjugated
    应用范围
    • 58
    • 35
    • 24
    • 18
    • 13
    • 13
    • 12
    • 10
    • 9
    • 4
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    原理
    Anti-nmt55/p54nrb/NONO Antibody Picoband®
    序列
    MQSNKTFNLE KQNHTPRKHH QHHHQQQHHQ QQQQQ
    交叉反应 (详细)
    No cross-reactivity with other proteins.
    产品特性
    Anti-nmt55/p54nrb/NONO Antibody Picoband® (ABIN3044530). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    A synthetic peptide corresponding to a sequence at the N-terminus of human nmt55/p54nrb, identical to the related mouse and rat sequences.
    亚型
    IgG
    Top Product
    Discover our top product NONO Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. "Entrez Gene: NONO Non-POU domain containing, octamer-binding". 2. Dong B, Horowitz DS, Kobayashi R, Krainer AR (Oct 1993). "Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6". Nucleic Acids Res. 21 (17): 4085-92. 3. Traish AM, Huang YH, Ashba J, Pronovost M, Pavao M, McAneny DB, Moreland RB (Dec 1997). "Loss of expression of a 55 kDa nuclear protein (nmt55) in estrogen receptor-negative human breast cancer". Diagn. Mol. Pathol. 6(4): 209-21.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原
    NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
    别名
    NONO (NONO 产品)
    背景

    Synonyms: Non-POU domain-containing octamer-binding protein,NonO protein,54 kDa nuclear RNA- and DNA-binding protein,55 kDa nuclear protein,DNA-binding p52/p100 complex, 52 kDa subunit,NMT55,p54 (nrb),p54nrb,NONO,NRB54,

    Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines. .

    Background: Non-POU domain-containing octamer-binding protein?is a?protein?that in humans is encoded by the?NONO gene. This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.?

    分子量
    60 kDa
    基因ID
    4841
    UniProt
    Q15233
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