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N-Cadherin 抗体 (Middle Region)

Name: N-Cadherin 抗体 (Middle Region)
SKU: ABIN3044370
Availability: OutOfStock

This anti-N-Cadherin antibody is a 兔多克隆 antibody detecting N-Cadherin in WB, IHC, IF 和 FACS. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 16+ publications.

Anti-N Cadherin antibody, Western blotting Lane 1: MCF-7 Cell Lysate Lane 2: HELA Cell Lysate (N-Cadherin 抗体 (Middle Region))

Anti-N Cadherin antibody, IHC(P) IHC(P): Human Ovary Cancer Tissue (N-Cadherin 抗体 (Middle Region))

IHC

2 images

(16 references)

产品编号 ABIN3044370

发货至: 中国

Datasheet as PDF

Quick Overview for N-Cadherin 抗体 (Middle Region) (ABIN3044370)

抗原

See all N-Cadherin (CDH2) 抗体

N-Cadherin (CDH2) (Cadherin 2 (CDH2))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This N-Cadherin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Discover our top product CDH2 Primary Antibody

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AA 701-714, Middle Region

原理

Anti-N-Cadherin-2 CDH2 CD325-Antibody Picoband®

序列

CQCDSNGDCT DVDR

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-N-Cadherin-2 CDH2 -Antibody (ABIN3044370). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human N Cadherin, identical to the related rat and mouse sequences.

亚型

IgG
anti-Cadherin 2 (CDH2) (AA 701-800) antibody
CDH2 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cadherin 2 (CDH2) antibody
CDH2 适用: 人, 小鼠, 犬, 猴 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 2G7 unconjugated

anti-Cadherin 2 (CDH2) (AA 750-850) antibody
CDH2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Cadherin 2 (CDH2) antibody
CDH2 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 5D5 unconjugated

anti-Cadherin 2 (CDH2) (AA 45-238) antibody
CDH2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cadherin 2 (CDH2) (AA 685-784) antibody
CDH2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cadherin 2 (CDH2) (AA 295-414) antibody
CDH2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cadherin 2 (CDH2) (Extracellular Domain) antibody
CDH2 适用: 人 WB, IHC, IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal 8C11 unconjugated

anti-Cadherin 2 (CDH2) antibody
CDH2 适用: 人 WB, IHC, IHC (f) 宿主: 兔 Monoclonal unconjugated Recombinant Antibody

anti-Cadherin 2 (CDH2) (C-Term) antibody
CDH2 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Miyatani, S., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Takeichi, M. : Genomic structure and chromosomal mapping of the mouse N-cadherin gene. Proc. Nat. Acad. Sci. 89: 8443-8447, 1992. 2. Walsh, F. S., Barton, C. H., Putt, W., Moore, S. E., Kelsell, D., Spurr, N., Goodfellow, P. N. : N-cadherin gene maps to human chromosome 18 and is not linked to the E-cadherin gene. J. Neurochem. 55: 805-812, 1990. 3. Togashi, H., Abe, K., Mizoguchi, A., Takaoka, K., Chisaka, O., Takeichi, M. : Cadherin regulates dendritic spine morphogenesis. Neuron 35: 77-89, 2002.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

有效期

12 months
Inoue, Takemori, Mizuguchi, Kimura, Chikugo, Hagiyama, Yoneshige, Mori, Maenishi, Kometani, Itoh, Satou, Ito: "Heart-bound adiponectin, not serum adiponectin, inversely correlates with cardiac hypertrophy in stroke-prone spontaneously hypertensive rats." in: Experimental physiology, Vol. 102, Issue 11, pp. 1435-1447, (2018) (PubMed).

Zhang, Bu, Sun, Xu, Yao, He, Lai: "Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 270, (2018) (PubMed).

Jia, Jiang, Liu, Wang, Zhu, Zhu, Liu, Zhong, Xie, Huang, Jia, Li, Liu, Zuo, Cheng, Dai, Ren: "Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells." in: International journal of oncology, Vol. 52, Issue 6, pp. 1787-1800, (2018) (PubMed).

Shen, Cai, Liu, Li, Gan, Li, Wang, Guo, Zhou, Lu, Sun, Li: "Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy." in: Journal of hematology & oncology, Vol. 11, Issue 1, pp. 95, (2018) (PubMed).

Pan, An, Meng, Li, Ren, Guan: "MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/β-catenin pathway." in: Experimental and therapeutic medicine, Vol. 14, Issue 5, pp. 4663-4670, (2017) (PubMed).

Wan, Tian, Qi, Liu, Xu: "ANXA1 affects cell proliferation, invasion and epithelial-mesenchymal transition of oral squamous cell carcinoma." in: Experimental and therapeutic medicine, Vol. 14, Issue 5, pp. 5214-5218, (2017) (PubMed).

Shan, Lv, Zhao, Liu, Sun, Xi, Xiao, Li: "Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 10, pp. 12357-67, (2016) (PubMed).

Lin, Sun, Wang, Zheng, Zhang, Zheng: "Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition." in: Frontiers in pharmacology, Vol. 6, pp. 320, (2016) (PubMed).

Tong, Sun, Wang, Suo: "Sorcin Enhances Metastasis and Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer." in: Cell biochemistry and biophysics, (2015) (PubMed).

Liu, Liu, Jiang, Peng, Ma, Lu: "SOX9 Overexpression Promotes Glioma Metastasis via Wnt/?-Catenin Signaling." in: Cell biochemistry and biophysics, (2015) (PubMed).

Sun, Hu, Zheng, Bagnoli, Guo, Rupaimoole, Rodriguez-Aguayo, Lopez-Berestein, Ji, Chen, Sood, Mezzanzanica, Liu, Sun, Zhang: "MiR-506 inhibits multiple targets in the epithelial-to-mesenchymal transition network and is associated with good prognosis in epithelial ovarian cancer." in: The Journal of pathology, Vol. 235, Issue 1, pp. 25-36, (2015) (PubMed).

Su, Li: "RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10760-7, (2015) (PubMed).

Yang, Hui, Wang, Yang, Jiang: "Overexpression of SOX2 promotes migration, invasion, and epithelial-mesenchymal transition through the Wnt/?-catenin pathway in laryngeal cancer Hep-2 cells." in: Tumour biology, Vol. 35, Issue 8, pp. 7965-73, (2014) (PubMed).

Kudo-Sakamoto, Akazawa, Ito, Takano, Yano, Yabumoto, Naito, Oka, Lee, Sakata, Suzuki, Saido, Komuro: "Calpain-dependent cleavage of N-cadherin is involved in the progression of post-myocardial infarction remodeling." in: The Journal of biological chemistry, Vol. 289, Issue 28, pp. 19408-19, (2014) (PubMed).

Wang, Li, Zhou, Sun, Wang, Cheng, Ma, Liu, Liu, Wang, Liang, Wu: "Intervention effects of ganoderma lucidum spores on epileptiform discharge hippocampal neurons and expression of neurotrophin-4 and N-cadherin." in: PLoS ONE, Vol. 8, Issue 4, pp. e61687, (2013) (PubMed).

Qiao, Johnson, Gao et al.: "Epithelial-mesenchymal transition in oral squamous cell carcinoma triggered by transforming growth factor-beta1 is Snail family-dependent and correlates with matrix metalloproteinase-2 and -9 ..." in: International journal of oncology, Vol. 37, Issue 3, pp. 663-8, (2010) (PubMed).
抗原

N-Cadherin (CDH2) (Cadherin 2 (CDH2))

别名

CDH2

背景

Synonyms: Cadherin-2,CDw325,Neural cadherin,N-cadherin,CD325,CDH2,CDHN, NCAD,
Background: N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96 % identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

分子量

140 kDa

UniProt

P19022

途径

Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane