Cullin 4B 抗体 (C-Term)
Quick Overview for Cullin 4B 抗体 (C-Term) (ABIN3044348)
抗原
See all Cullin 4B (CUL4B) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 895-913, C-Term
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原理
- Anti-Cullin 4B/CUL4B Antibody Picoband®
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序列
- DRDYMERDKE NPNQYNYIA
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Cullin 4B/CUL4B Antibody (ABIN3044348). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human Cullin 4B, identical to the related rat and mouse sequences.
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亚型
- IgG
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anti-Cullin 4B (CUL4B) (Internal Region) antibody
CUL4B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cullin 4B (CUL4B) (AA 1-300) antibodyCUL4B 适用: 人, 小鼠, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1C4 unconjugated
anti-Cullin 4B (CUL4B) (AA 1-913) antibodyCUL4B 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Cullin 4B (CUL4B) antibodyCUL4B 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cullin 4B (CUL4B) (AA 1-300) antibodyCUL4B 适用: 人, 小鼠, 大鼠, 犬, 猴 WB 宿主: 小鼠 Monoclonal 7F8 unconjugated
anti-Cullin 4B (CUL4B) antibodyCUL4B 适用: 人, 大鼠, 斑马鱼, 鱼 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cullin 4B (CUL4B) (AA 250-278) antibodyCUL4B 适用: 人 WB 宿主: 兔 Polyclonal RB42521 unconjugated
anti-Cullin 4B (CUL4B) (C-Term) antibodyCUL4B 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cullin 4B (CUL4B) (AA 1-200) antibodyCUL4B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cullin 4B (CUL4B) (N-Term) antibodyCUL4B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Zebrafish
Western blot, 0.1-0.5 μg/mL, Human, Rat, Zebrafish, Mouse
1. Zou, Y., Liu, Q., Chen, B., Zhang, X., Guo, C., Zhou, H., Li, J., Gao, G., Guo, Y., Yan, C., Wei, J., Shao, C., Gong, Y. : Mutation in CUL4B, which encodes a member of cullin-RING ubiquitin ligase complex, causes X-linked mental retardation. Am. J. Hum. Genet. 80: 561-566, 2007. 2. Tarpey, P. S., Raymond, F. L., O'Meara, S., Edkins, S., Teague, J., Butler, A., Dicks, E., Stevens, C., Tofts, C., Avis, T., Barthorpe, S., Buck, G. : {and 41 others}: Mutations in CUL4B, which encodes a ubiquitin E3 ligase subunit, cause an X-linked mental retardation syndrome associated with aggressive outbursts, seizures, relative macrocephaly, central obesity, hypogonadism, pes cavus, and tremor. Am. J. Hum. Genet. 80: 345-352, 2007. 3. Ohtake, F., Baba, A., Takada, I., Okada, M., Iwasaki, K., Miki, H., Takahashi, S., Kouzmenko, A., Nohara, K., Chiba, T., Fujii-Kuriyama, Y., Kato, S. : Dioxin receptor is a ligand-dependent E3 ubiquitin ligase. Nature 446: 562-566, 2007. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Cullin 4B (CUL4B)
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别名
- CUL4B
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背景
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Synonyms: Cullin-4B,CUL-4B,CUL4B,KIAA0695,
Tissue Specificity: Bcl-X (S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X (L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Background: Cullin 4B/CUL4B encodes a scaffold protein that organizes a cullin-RING (really interesting new gene) ubiquitin ligase (E3) complex in ubiquitylation. The CUL4 gene encodes a protein of 913 amino acids. The cullin domain is located between amino acid residues 217 and 815 and is characterized a by C-terminal globular domain (cullin homology domain) and a series of N-terminal repeats (cullin repeats). Ohtake et al. (2007) characterize a fat-soluble ligand-dependent ubiquitin ligase complex in human cell lines, in which dioxin receptor (AhR) is integrated as a component of a novel cullin 4B ubiquitin ligase complex, CUL4B (AhR). Complex assembly and ubiquitin ligase activity of CUL4B (AhR) in vitro and in vivo are dependent on the AhR ligand. In the CUL4B (AhR) complex, ligand-activated AhR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Their findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex.
Sequence Similarities: Belongs to the cullin family.
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分子量
- 104 kDa
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UniProt
- Q13620
抗原
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