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BRCA1 抗体 (AA 1661-1863)

BRCA1 适用: 人, 大鼠, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3044313
发货至: 中国
  • 抗原 See all BRCA1 抗体
    BRCA1 (Breast Cancer 1 (BRCA1))
    抗原表位
    • 22
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    AA 1661-1863
    适用
    • 204
    • 38
    • 27
    • 3
    • 2
    • 1
    • 1
    • 1
    人, 大鼠, 小鼠
    宿主
    • 175
    • 39
    • 2
    克隆类型
    • 179
    • 37
    多克隆
    标记
    • 92
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    This BRCA1 antibody is un-conjugated
    应用范围
    • 124
    • 61
    • 54
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    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
    原理
    Anti-BRCA1 Antibody Picoband®
    交叉反应 (详细)
    No cross-reactivity with other proteins
    产品特性
    Anti-BRCA1 Antibody Picoband® (ABIN3044313). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    E.coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequences identity with mouse and rat BRCA1, respectively.
    亚型
    IgG
    Top Product
    Discover our top product BRCA1 Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL, Human
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. Lee, M.-H., Lahusen, T., Wang, R.-H., Xiao, C., Xu, X., Hwang, Y.-S., He, W.-W., Shi, Y., Deng, C.-X. Yin Yang 1 positively regulates BRCA1 and inhibits mammary cancer formation. Oncogene 31: 116-127, 2012. 2. Thompson, M. E., Jensen, R. A., Obermiller, P. S., Page, D. L., Holt, J. T. Decreased expression of BRCA1 accelerates growth and is often present during sporadic breast cancer progression. Nature Genet. 9: 444-450, 1995. 3. Wang, B., Hurov, K., Hofmann, K., Elledge, S. J. NBA1, a new player in the Brca1 A complex, is required for DNA damage resistance and checkpoint control. Genes Dev. 23: 729-739, 2009.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Liu, Lai, Li, Sun, Cheng, Ge, Wang, Li, Zhang, De Felici, Dyce, Shen: "Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro." in: Cell death & disease, Vol. 8, Issue 8, pp. e2966, (2017) (PubMed).

    Zhang, Zhang, Cong, Zhang: "The ectopic expression of BRCA1 is associated with genesis, progression, and prognosis of breast cancer in young patients." in: Diagnostic pathology, Vol. 7, pp. 181, (2013) (PubMed).

  • 抗原
    BRCA1 (Breast Cancer 1 (BRCA1))
    别名
    BRCA1 (BRCA1 产品)
    背景

    Synonyms: Breast cancer type 1 susceptibility protein,6.3.2.-,RING finger protein 53,BRCA1,RNF53,

    Tissue Specificity: Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.

    Background: BRCA1, also known as BRCC1, is a gene which mapping to 17q21.3. This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by mutation or by alterations in gene expression.

    Sequence Similarities: Contains 2 BRCT domains.

    分子量
    290 kDa
    基因ID
    672
    UniProt
    P38398
    途径
    Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus
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