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Hsc70 抗体 (N-Term)

HSPA8 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

Lane 9: COLO320 Cell Lysate (Hsc70 抗体 (N-Term))

Anti-Hsc70 antibody, ICC ICC: HELA Cell (Hsc70 抗体 (N-Term))

Anti-Hsc70 antibody, IHC(F) IHC(F): Rat Intestine Tissue (Hsc70 抗体 (N-Term))

Anti-Hsc70 antibody, IHC(P) IHC(P): Rat Brain Tissue (Hsc70 抗体 (N-Term))

Anti-Hsc70 antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue (Hsc70 抗体 (N-Term))

5 images

(5 references)

产品编号 ABIN3044300

发货至: 中国

Datasheet as PDF

抗原 See all Hsc70 (HSPA8) 抗体

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
抗原表位
20

7

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 13-31, N-Term
适用
81

42

37

17

14

7

6

4

4

4

4

4

4

3

3

2

2

2

1

1

1

1

1
人, 大鼠, 小鼠
宿主
82

28

4

2
兔
Compatible Secondaries
克隆类型
78

37
多克隆
标记
67

17

15

4

2

1

1

1

1

1

1

1

1

1

1

1
This Hsc70 antibody is un-conjugated
应用范围
103

71

52

38

36

35

17

9

9

9

9

4

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

原理

Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband®

序列

TTYSCVGVFQ HGKVEIIAN

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-Hsp70/HSPA1A/HSPA1B Antibody (ABIN3044300). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human Hsp70, identical to the related rat and mouse sequence.

亚型

IgG

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anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 6E4D2 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IP, FACS 宿主: 小鼠 Monoclonal 2G8F6 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠, Cow, 绵羊, Pig, 斑马鱼, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibody
HSPA8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (Internal Region) antibody
Verified HSPA8 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody (HRP)
HSPA8 适用: 人 WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA 宿主: 小鼠 Monoclonal 1F2-H5 HRP

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody (FITC)
HSPA8 适用: 人 WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA 宿主: 小鼠 Monoclonal 1F2-H5 FITC

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody (Biotin)
HSPA8 适用: 人 WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA 宿主: 小鼠 Monoclonal 1F2-H5 Biotin

应用备注

Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Tavaria M, Gabriele T, Kola I, Anderson RL (April 1996). "A hitchhiker's guide to the human Hsp70 family". Cell Stress Chaperones 1 (1): 23-8. 2. Morano KA (October 2007). "New tricks for an old dog: the evolving world of Hsp70". Ann. N. Y. Acad. Sci. 1113: 1-14. 3. Luders, J., Demand, J., Hohfeld, J. (2000), Journal of Biological Chemistry 275 (7): 4613-461, http://www.jbc.org/cgi/content/full/275/7/4613, retrieved on 2009-04-07

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Zhang, Tang, Li, Li, Zhang, Yan, Cui: "Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 5371-8, (2016) (PubMed).

Pu, Shi, Wang, Wang, Guo, Liu, Sun, Gao, Zhou: "Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats." in: Journal of biomedical research, Vol. 25, Issue 4, pp. 292-8, (2013) (PubMed).

Li, Roubeix, Wang, Shi, Liu, Baudouin, Chen: "Therapeutic efficacy of trehalose eye drops for treatment of murine dry eye induced by an intelligently controlled environmental system." in: Molecular vision, Vol. 18, pp. 317-29, (2012) (PubMed).

Yi, Peng, Chang, Peng, Yan, Lin: "Effect of pre-moxibustion on apoptosis and proliferation of gastric mucosa cells." in: World journal of gastroenterology, Vol. 13, Issue 15, pp. 2174-8, (2007) (PubMed).

Wu, Cao, Gao, Chen, Wang, Zumbika, Qian: "Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells." in: World journal of gastroenterology, Vol. 10, Issue 20, pp. 2944-8, (2004) (PubMed).
抗原

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

别名

HSPA8 (HSPA8 产品)

背景

Synonyms: Heat shock 70 kDa protein 1A; Heat shock 70 kDa protein 1B
Tissue Specificity: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). .
Background: The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP (Adenosine triphosphate) and hydrolyzes it to ADP (Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. By binding tightly to partially-synthesized peptide sequences (incomplete proteins), Hsp70 prevents them from aggregating and being rendered nonfunctional. And it also can act to protect cells from thermal or oxidative stress. Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins. Interaction with CHIP (Carboxyl-terminus of Hsp70 Interacting Protein)–an E3 ubiquitin ligase–allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.
Sequence Similarities: Belongs to the beta-catenin family.

分子量

70 kDa

UniProt

P08P09

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