CAPNL1 抗体 (Middle Region)
(3 references)-
- 抗原 See all CAPNL1 抗体
- CAPNL1 (Calpain 1, Large Subunit (CAPNL1))
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抗原表位
- AA 312-326, Middle Region
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CAPNL1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 原理
- Anti-Calpain 1/CAPN1 Antibody Picoband®
- 序列
- EWNNVDPYER DQLRV
- 交叉反应 （详细）
- No cross-reactivity with other proteins
- 产品特性
- Anti-Calpain 1/CAPN1 Antibody (ABIN3044292). Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1, different from the mouse sequence by two amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product CAPNL1 Primary Antibody
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anti-Calpain 1, Large Subunit (CAPNL1) (AA 501-714) antibody
CAPNL1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 5B2G10 unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 501-714) antibodyCAPNL1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1E6G9 unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 20-330), (Catalytic Subunit) antibodyCAPNL1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 1-714), (Catalytic Subunit) antibodyCAPNL1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 1-420) antibodyCAPNL1 适用: 人 WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1376CT809-24-85 unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 1-714) antibodyCAPNL1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 1-714) antibodyCAPNL1 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 1-714) antibodyCAPNL1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) antibodyCAPNL1 适用: 人 ELISA, IHC, Coat, StM 宿主: 小鼠 Monoclonal CAPN1-1530 unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) antibodyCAPNL1 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human, Mouse
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Aoki K, Imajoh S, Ohno S, Emori Y, Koike M, Kosaki G, Suzuki K (Oct 1986). "Complete amino acid sequence of the large subunit of the low-Ca2+-requiring form of human Ca2+-activated neutral protease (muCANP) deduced from its cDNA sequence". - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. - 有效期
- 12 months
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: "Over-expression of calpastatin inhibits calpain activation and attenuates post-infarction myocardial remodeling." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120178, (2015) (PubMed).
: "Fibrosis, adipogenesis, and muscle atrophy in congenital muscular torticollis." in: Medicine, Vol. 93, Issue 23, pp. e138, (2014) (PubMed).
: "Suppression of NHE1 by small interfering RNA inhibits HIF-1?-induced angiogenesis in vitro via modulation of calpain activity." in: Microvascular research, Vol. 81, Issue 2, pp. 160-8, (2011) (PubMed).
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: "Over-expression of calpastatin inhibits calpain activation and attenuates post-infarction myocardial remodeling." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120178, (2015) (PubMed).
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- 抗原
- CAPNL1 (Calpain 1, Large Subunit (CAPNL1))
- 别名
- CAPN1 (CAPNL1 产品)
- 背景
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Synonyms: Calpain-1 catalytic subunit,3.4.22.52,Calcium-activated neutral proteinase 1,CANP 1,Calpain mu-type,Calpain-1 large subunit,Cell proliferation-inducing gene 30 protein,Micromolar-calpain,muCANP,CAPN1,CANPL1,PIG30,
Tissue Specificity: Ubiquitous.
Background: Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I (mu-calpain) and calpain II (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 80 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1 (CAPN1) and L2. By quantitative RT-PCR, Ueyama et al. (1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy (e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis (ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al. (1989, 1990) assign CANPL1 to chromosome 11.
Sequence Similarities: Belongs to the peptidase C2 family.
- 分子量
- 82 kDa
- UniProt
- P07384
- 途径
- Apoptosis
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