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CYBB 抗体 (Middle Region)

Name: CYBB 抗体 (Middle Region)
SKU: ABIN3044252
Availability: OutOfStock

CYBB 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

Anti-NOX2/gp91phox antibody, Western blotting Lane 1: HELA Cell Lysate Lane 2: JURKAT Cell Lysate Lane 3: MCF-7 Cell Lysate Lane 4: SMMC Cell Lysate (CYBB 抗体 (Middle Region))

Anti-NOX2/gp91phox antibody, IHC(P) IHC(P): Human Intestinal Cancer Tissue (CYBB 抗体 (Middle Region))

IHC

2 images

(7 references)

产品编号 ABIN3044252

发货至: 中国

Datasheet as PDF

抗原 See all CYBB 抗体

CYBB (Cytochrome B-245, beta Polypeptide (CYBB))
抗原表位
15

6

4

3

1

1

1

1

1

1

1

1

1

1
AA 442-459, Middle Region
适用
55

37

36

19

15

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
51

4

2
兔
Compatible Secondaries
克隆类型
43

14
多克隆
标记
24

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This CYBB antibody is un-conjugated
应用范围
35

19

16

12

12

7

6

6

3

3

3
Western Blotting (WB), Immunohistochemistry (IHC)

原理

Anti-NOX2/gp91phox/CYBB Antibody Picoband®

序列

YWLCRDTHAF EWFADLLQ

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-NOX2/gp91phox/CYBB Antibody (ABIN3044252). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human NOX2, identical to the related rat and mouse sequences.

亚型

IgG

Top Product

Discover our top product CYBB Primary Antibody
anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 501-570) antibody
CYBB 适用: 人, 小鼠, 大鼠, 兔, 小鸡 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 283-570) antibody
CYBB 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (C-Term) antibody
CYBB 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) antibody
CYBB 适用: 人 WB, IF, IHC (p), ICC 宿主: 小鼠 Monoclonal 54-1 unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 283-570) antibody
CYBB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (Internal Region) antibody
Verified CYBB 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 100-200) antibody
CYBB 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 416-500) antibody
CYBB 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 501-570) antibody (Biotin)
CYBB 适用: 人, 小鼠, 大鼠, 兔, 小鸡 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal Biotin

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 501-570) antibody (AbBy Fluor® 350)
CYBB 适用: 人, 小鼠, 大鼠, 兔, 小鸡 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 350

应用备注

Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
1. Bionda, C., Li, X. J., van Bruggen, R., Eppink, M., Roos, D., Morel, F., Stasia, M.-J. Functional analysis of two-amino acid substitutions in gp91phox in a patient with X-linked flavocytochrome b(558)-positive chronic granulomatous disease by means of transgenic PLB-985 cells. Hum. Genet. 115: 418-427, 2004. 2. Bolscher, B. G. J. M., de Boer, M., de Klein, A., Weening, R. S., Roos, D. Point mutations in the beta-subunit of cytochrome b(558) leading to X-linked chronic granulomatous disease. Blood 77: 2482-2487, 1991. 3. "Entrez Gene: CYBB cytochrome b-245, beta polypeptide (chronic granulomatous disease)".

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

有效期

12 months
Zhang, Deng, Lai, Guan, Sun, Han, Wang, Pan, Ji, Luo, Huang, Tang, Gu, Dan, Yu, Namaka, Zhang, Deng, Li: "Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation." in: Scientific reports, Vol. 6, pp. 30146, (2018) (PubMed).

Deep, Kumar, Jain, Dhar, Panigrahi, Hussain, Agarwal, El-Elimat, Sica, Oberlies, Agarwal: "Graviola inhibits hypoxia-induced NADPH oxidase activity in prostate cancer cells reducing their proliferation and clonogenicity." in: Scientific reports, Vol. 6, pp. 23135, (2017) (PubMed).

Meyer, Fredette, Daniel, Sharma, Amann, Arterburn, Barton, Prossnitz: "Obligatory role for GPER in cardiovascular aging and disease." in: Science signaling, Vol. 9, Issue 452, pp. ra105, (2017) (PubMed).

Wang, Wang, Liu, Wang, Zhao, Wang, Yin: "Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells." in: Carbohydrate polymers, Vol. 125, pp. 135-45, (2015) (PubMed).

Du, Yang, Liu, Li, Zhao, Hu, Liu, Qian, Gao: "NADPH oxidase 2-dependent oxidative stress, mitochondrial damage and apoptosis in the ventral cochlear nucleus of D-galactose-induced aging rats." in: Neuroscience, Vol. 286, pp. 281-92, (2015) (PubMed).

Du, Yang, Zhou, Liu, Li, Chen, Gao: "D?galactose?induced mitochondrial DNA oxidative damage in the auditory cortex of rats." in: Molecular medicine reports, Vol. 10, Issue 6, pp. 2861-7, (2014) (PubMed).

Liao, Liu, Guo, Zhang, Zhang: "Oxidative burst of circulating neutrophils following traumatic brain injury in human." in: PLoS ONE, Vol. 8, Issue 7, pp. e68963, (2013) (PubMed).
抗原

CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

别名

CYBB (CYBB 产品)

背景

Synonyms: Cytochrome b-245 heavy chain,1.-.-.-,CGD91-phox,Cytochrome b (558) subunit beta,Cytochrome b558 subunit beta,Heme-binding membrane glycoprotein gp91phox,NADPH oxidase 2,Neutrophil cytochrome b 91 kDa polypeptide,Superoxide-generating NADPH oxidase heavy chain subunit,gp91-1,gp91-phox,p22 phagocyte B-cytochrome,CYBB,NOX2,
Tissue Specificity: Detected in neutrophils (at protein level). .
Background: NOX2 (NADPH OXIDASE 2), also called CYBB (CYTOCHROME b (-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).
Sequence Similarities: Contains 1 FAD-binding FR-type domain.

分子量

65 kDa

UniProt

P04839