MPG 抗体 (C-Term)
Quick Overview for MPG 抗体 (C-Term) (ABIN3044195)
抗原
See all MPG 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 228-243, C-Term
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原理
- Anti-MPG Antibody Picoband®
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序列
- NKSFDQRDLA QDEAVW
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-MPG Antibody (ABIN3044195). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human MPG, different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid.
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亚型
- IgG
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anti-N-Methylpurine-DNA Glycosylase (MPG) (AA 1-90) antibody
MPG 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E10 unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (AA 221-296) antibodyMPG 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (AA 1-90) antibodyMPG 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1G6 unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (C-Term) antibodyVerified MPG 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (C-Term) antibodyMPG 适用: 人, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (C-Term) antibodyMPG 适用: 人, 大鼠, 小鼠, Cow, 豚鼠, 兔, Hamster, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) antibodyMPG 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (C-Term) antibodyMPG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (AA 46-315) antibodyMPG 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (AA 32-300) antibodyMPG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Boosalis, M., Derfler, B., Call, K., Samson, L. Cloning of a human 3-methyladenine DNA glycosylase cDNA whose gene maps to human chromosome 16. (Abstract) Am. J. Hum. Genet. 49 (suppl.): 399 only, 1991. 2. Engelward, B. P., Weeda, G., Wyatt, M. D., Broekhof, J. L. M., de Wit, J., Donker, I., Allan, J. M., Gold, B., Hoeijmakers, J. H. J., Samson, L. D.Base excision repair deficient mice lacking the Aag alkyladenine DNA glycosylase. Proc. Nat. Acad. Sci. 94: 13087-13092, 1997. 3. Kielman, M. F., Smits, R., Bernini, L. F. Localization and characterization of the mouse alpha-globin locus control region. Genomics 21: 431-433, 1994. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- MPG (N-Methylpurine-DNA Glycosylase (MPG))
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别名
- MPG
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背景
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Synonyms: DNA-3-methyladenine glycosylase;3.2.2.21;3-alkyladenine DNA glycosylase;3-methyladenine DNA glycosidase;ADPG;N-methylpurine-DNA glycosylase;MPG;AAG, ANPG, MID1;
Tissue Specificity: Expressed in breast, ovary and prostate.
Background: MPG (N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The MPG gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line (HT29). The completely characterized human MPG gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo, Hang et al. (1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.
Sequence Similarities: Belongs to the DNA glycosylase MPG family.
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分子量
- 33 kDa
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UniProt
- P29372
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途径
- DNA Damage Repair
抗原
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