MPG 抗体 (C-Term)
Quick Overview for MPG 抗体 (C-Term) (ABIN3044195)
抗原
See all MPG 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 228-243, C-Term
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原理
- Anti-MPG Antibody
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序列
- NKSFDQRDLA QDEAVW
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-MPG Antibody (ABIN3044195). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human MPG, different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid.
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亚型
- IgG
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anti-N-Methylpurine-DNA Glycosylase (MPG) (AA 1-90) antibody
MPG 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E10 unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (AA 221-296) antibodyMPG 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (AA 1-90) antibodyMPG 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1G6 unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (C-Term) antibodyVerified MPG 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (C-Term) antibodyMPG 适用: 人, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (C-Term) antibodyMPG 适用: 人, 大鼠, 小鼠, Cow, 豚鼠, 兔, Hamster, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) antibodyMPG 适用: 人 WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (C-Term) antibodyMPG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (AA 46-315) antibodyMPG 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-N-Methylpurine-DNA Glycosylase (MPG) (AA 32-300) antibodyMPG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
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说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- MPG (N-Methylpurine-DNA Glycosylase (MPG))
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别名
- MPG
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背景
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Synonyms: DNA-3-methyladenine glycosylase;3.2.2.21;3-alkyladenine DNA glycosylase;3-methyladenine DNA glycosidase;ADPG;N-methylpurine-DNA glycosylase;MPG;AAG, ANPG, MID1;
Tissue Specificity: Expressed in breast, ovary and prostate.
Background: MPG (N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The MPG gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line (HT29). The completely characterized human MPG gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo, Hang et al. (1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.
Sequence Similarities: Belongs to the DNA glycosylase MPG family.
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分子量
- 33 kDa
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UniProt
- P29372
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途径
- DNA Damage Repair
抗原
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