E2F6 抗体 (Middle Region)
-
- 抗原 See all E2F6 抗体
- E2F6 (E2F Transcription Factor 6 (E2F6))
-
抗原表位
- AA 162-177, Middle Region
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This E2F6 antibody is un-conjugated
-
应用范围
- Western Blotting (WB)
- 原理
- Rabbit IgG polyclonal antibody for Transcription factor E2F6(E2F6) detection. Tested with WB in Human,Mouse,Rat.
- 序列
- KDCAQQLFEL TDDKEN
- 交叉反应 (详细)
-
Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
-
Rabbit IgG polyclonal antibody for Transcription factor E2F6(E2F6) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: E2F transcription factor 6
Protein Name: Transcription factor E2F6(E2F-6) - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human E2F6(162-177aa KDCAQQLFELTDDKEN), different from the related mouse and rat sequences by one amino acid.
- 亚型
- IgG
- Top Product
- Discover our top product E2F6 Primary Antibody
-
-
- 应用备注
-
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
-
- 抗原
- E2F6 (E2F Transcription Factor 6 (E2F6))
- 别名
- E2F6 (E2F6 产品)
- 别名
- E2F-6 antibody, AI462434 antibody, E2F6b antibody, EMA antibody, E2F transcription factor 6 antibody, E2F6 antibody, E2f6 antibody
- 背景
-
E2F6(E2F transcription factor 6) also known as E2F-6. Northern blot analysis revealed that E2F6 is expressed as 2 mRNAs, 2.5 and 3.5 kb, in all human tissues and cell lines tested. The predicted human and mouse E2F6 protein sequences are 92 % identical. The DNA-binding and dimerization domains of E2F6 are highly related to those of other E2F family members, but this protein lacks the sequences necessary for either transcriptional activation or binding to RB1, p107, or p130. E2F6 can act to repress the transcription of E2F-responsive genes by countering the activity of other E2F complexes. E2F6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. E2F6 is found in a multimeric protein complex that contains MGA and MAX, and thus the complex can bind not only to the E2F binding site but also to MYC- and Brachyury- binding sites. The E2F6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells.
Synonyms: E2F 6 antibody|E2F binding site modulating activity protein antibody|E2F transcription factor 6 antibody|E2F transcription factor 6 isoform 1 antibody|E2F-6 antibody|E2F6 antibody|E2F6_HUMAN antibody|EMA antibody|MGC111545 antibody|Transcription factor E2F6 antibody - UniProt
- O75461
-