E2F6 抗体 (Middle Region)
Quick Overview for E2F6 抗体 (Middle Region) (ABIN3044189)
抗原
See all E2F6 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 162-177, Middle Region
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原理
- Anti-Transcription factor E2F6 E2F6 Antibody Picoband®
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序列
- KDCAQQLFEL TDDKEN
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Transcription factor E2F6 E2F6 Antibody (ABIN3044189). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human E2F6, different from the related mouse and rat sequences by one amino acid.
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亚型
- IgG
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anti-E2F Transcription Factor 6 (E2F6) (Internal Region) antibody
E2F6 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 6 (E2F6) (AA 1-281) antibodyE2F6 适用: 人 WB, IF, IP, ChIP 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 6 (E2F6) antibodyE2F6 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 6 (E2F6) (AA 1-281) antibodyE2F6 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1F7-A9 unconjugated
anti-E2F Transcription Factor 6 (E2F6) antibodyE2F6 适用: 人 WB, IP, ChIP 宿主: 兔 Monoclonal unconjugated
anti-E2F Transcription Factor 6 (E2F6) antibodyE2F6 适用: 人, 小鼠 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 6 (E2F6) (AA 1-281) antibodyE2F6 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 6 (E2F6) (Internal Region) antibodyE2F6 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 6 (E2F6) (AA 1-281) antibodyE2F6 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 6 (E2F6) (AA 43-92) antibodyE2F6 适用: 人, 小鼠, Pig, 犬, 豚鼠, 马, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Ogawa, H., Ishiguro, K., Gaubatz, S., Livingston, D. M., Nakatani, Y. A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells. Science 296: 1132-1136, 2002. 2. Trimarchi, J. M., Fairchild, B., Verona, R., Moberg, K., Andon, N., Lees, J. A. E2F-6, a member of the E2F family that can behave as a transcriptional repressor. Proc. Nat. Acad. Sci. 95: 2850-2855, 1998. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- E2F6 (E2F Transcription Factor 6 (E2F6))
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别名
- E2F6
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背景
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Synonyms: Transcription factor E2F6;E2F-6;E2F6;
Tissue Specificity: Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
Background: E2F6 (E2F transcription factor 6) also known as E2F-6. Northern blot analysis revealed that E2F6 is expressed as 2 mRNAs, 2.5 and 3.5 kb, in all human tissues and cell lines tested. The predicted human and mouse E2F6 protein sequences are 92% identical. The DNA-binding and dimerization domains of E2F6 are highly related to those of other E2F family members, but this protein lacks the sequences necessary for either transcriptional activation or binding to RB1, p107, or p130. E2F6 can act to repress the transcription of E2F-responsive genes by countering the activity of other E2F complexes. E2F6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. E2F6 is found in a multimeric protein complex that contains MGA and MAX, and thus the complex can bind not only to the E2F binding site but also to MYC- and Brachyury- binding sites. The E2F6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells.
Sequence Similarities: Belongs to the E2F/DP family.
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分子量
- 32 kDa
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UniProt
- O75461
抗原
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