MCM7 抗体 (C-Term)
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Quick Overview for MCM7 抗体 (C-Term) (ABIN3044178)
抗原
See all MCM7 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Anti-MCM7 Antibody
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序列
- DEYEELNVWQ VNASRTRITF V
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特异性
- No cross reactivity with other proteins.
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交叉反应 (详细)
- No cross-reactivity with other proteins
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产品特性
- Anti-MCM7 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human MCM7.
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亚型
- IgG
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应用备注
- Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
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说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))
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别名
- MCM7
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背景
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Background: MCM7 (Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped on 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. Petrocca et al. (2008) found that MCM7 and the precursors of microRNAs (miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.
Gene Full Name: minichromosome maintenance complex component 7
Sequence Similarities: Belongs to the MCM family.
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分子量
- 80 kDa
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基因ID
- 4176
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UniProt
- P33993
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途径
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
抗原
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