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MBD4 抗体 (C-Term)

MBD4 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3044062
发货至: 中国
  • 抗原 See all MBD4 抗体
    MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))
    抗原表位
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 566-580, C-Term
    适用
    • 14
    • 7
    • 4
    • 1
    人, 小鼠, 大鼠
    宿主
    • 15
    • 1
    克隆类型
    • 16
    多克隆
    标记
    • 16
    This MBD4 antibody is un-conjugated
    应用范围
    • 14
    • 7
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    原理
    Anti-MBD4 Antibody Picoband®
    序列
    YHDWLWENHE KLSLS
    交叉反应 (详细)
    No cross-reactivity with other proteins
    产品特性
    Anti-MBD4 Antibody (ABIN3044062). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4, identical to the related rat and mouse sequences.
    亚型
    IgG
    Top Product
    Discover our top product MBD4 Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
    1. Hendrich, B., Abbott, C., McQueen, H., Chambers, D., Cross, S., Bird, A. Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes. Mammalian Genome 10: 906-912, 1999. 2. Hendrich, B., Bird, A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Molec. Cell. Biol. 18: 6538-6547, 1998. 3. Riccio, A., Aaltonen, L. A., Godwin, A. K., Loukola, A., Percesepe, A., Salovaara, R., Masciullo, V., Genuardi M., Paravatou-Petsotas, M., Bassi, D. E., Ruggeri, B. A., Klein-Szanto, A. J. P., Testa, J. R., Neri, G., Bellacosa, A. The DNA repair gene MBD4 (MED1) is mutated in human carcinomas with microsatellite instability. (Letter) Nature Genet. 23: 266-268, 1999.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    储存液
    Thimerosal (Merthiolate), Sodium azide
    注意事项
    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))
    别名
    MBD4 (MBD4 产品)
    背景

    Synonyms: Methyl-CpG-binding domain protein 4,3.2.2.-,Methyl-CpG-binding endonuclease 1,Methyl-CpG-binding protein MBD4,Mismatch-specific DNA N-glycosylase,MBD4,MED1,

    Tissue Specificity: Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes. .

    Background: MBD4 (Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells (Hendrich and Bird, 1998). Riccio et al. (1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH. Hendrich and Bird (1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al. (1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.

    Sequence Similarities: Contains 1 MBD (methyl-CpG-binding) domain.

    分子量
    80 kDa
    UniProt
    O95243
    途径
    DNA Damage Repair
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