WISP1 抗体 (C-Term)
Quick Overview for WISP1 抗体 (C-Term) (ABIN3043974)
抗原
See all WISP1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 354-367, C-Term
-
原理
- Anti-WISP1 Antibody Picoband®
-
序列
- ADLESYPDFS EIAN
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-WISP1 Antibody (ABIN3043974). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human WISP1, different from the related mouse and rat sequences by one amino acid.
-
亚型
- IgG
-
-
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (C-Term) antibody
Verified WISP1 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 213-350) antibodyWISP1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 130-251) antibodyWISP1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 1-280) antibodyWISP1 适用: 人 WB, IHC (p), IF 宿主: 小鼠 Polyclonal unconjugated
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 237-295) antibodyWISP1 适用: 小鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 216-347) antibodyWISP1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (C-Term) antibodyWISP1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 158-367) antibodyWISP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 216-347) antibodyWISP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 171-200) antibodyWISP1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB2115-2116 unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
1. Pennica, D., Swanson, T. A., Welsh, J. W., Roy, M. A., Lawrence, D. A., Lee, J., Brush, J., Taneyhill, L. A., Deuel, B., Lew, M., Watanabe, C., Cohen, R. L., Melhem, M. F., Finley, G. G., Quirke, P., Goddard, A. D., Hillan, K. J., Gurney, A. L., Botstein, D., Levine, A. J. WISP genes are members of the connective tissue growth factor family that are up-regulated in Wnt-1-transformed cells and aberrantly expressed in human colon tumors. Proc. Nat. Acad. Sci. 95: 14717-14722, 1998. 2. Tanaka, S., Sugimachi, K., Saeki, H., Kinoshita, J., Ohga, T., Shimada, M., Maehara, Y., Sugimachi, K. A novel variant of WISP1 lacking a von Willebrand type C module overexpressed in scirrhous gastric carcinoma. Oncogene 20: 5525-5532, 2001. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
-
-
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
-
别名
- WISP1
-
背景
-
Synonyms: WNT1-inducible-signaling pathway protein 1,WISP-1,CCN family member 4,Wnt-1-induced secreted protein,WISP1,CCN4,
Tissue Specificity: Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma.
Background: WISP1 (WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed (Tanaka et al., 2001). By use of radiation hybrid mapping panels, Pennica et al. (1998) mapped the WISP1 to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. Pennica et al. (1998) found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84 % of tumors examined compared with patient-matched normal mucosa.
Sequence Similarities: Belongs to the CCN family.
-
分子量
- 45 kDa
-
UniProt
- O95388
-
途径
- WNT signaling, Growth Factor Binding
抗原
-
