Peroxiredoxin 3 抗体 (AA 110-256)
Quick Overview for Peroxiredoxin 3 抗体 (AA 110-256) (ABIN3043904)
抗原
See all Peroxiredoxin 3 (PRDX3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 110-256
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原理
- Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband® (ABIN3043904). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Peroxiredoxin 3 recombinant protein (Position: T110-Q256). Human Peroxiredoxin 3 shares 93% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 3.
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亚型
- IgG
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anti-Peroxiredoxin 3 (PRDX3) (AA 1-256) antibody
PRDX3 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Peroxiredoxin 3 (PRDX3) (AA 5-233) antibodyPRDX3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Peroxiredoxin 3 (PRDX3) (AA 63-256) antibodyPRDX3 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Peroxiredoxin 3 (PRDX3) (AA 1-256) antibodyPRDX3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Peroxiredoxin 3 (PRDX3) (AA 239-256) antibodyPRDX3 适用: 人, 大鼠, 小鼠 WB, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Peroxiredoxin 3 (PRDX3) (AA 110-256) antibodyPRDX3 适用: 人, 大鼠, 小鼠 WB, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Peroxiredoxin 3 (PRDX3) (AA 1-256) antibodyPRDX3 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1E3-B2 unconjugated
anti-Peroxiredoxin 3 (PRDX3) antibodyPRDX3 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Peroxiredoxin 3 (PRDX3) antibodyPRDX3 适用: 人, 大鼠, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Peroxiredoxin 3 (PRDX3) (AA 239-256), (C-Term) antibodyPRDX3 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells1. Chiribau, C. B., Cheng, L., Cucoranu, I. C., Yu, Y.-S., Clempus, R. E., Sorescu, D. FOXO3A regulates peroxiredoxin III expression in human cardiac fibroblasts. J. Biol. Chem. 283: 8211-8217, 2008. 2. Nemoto, Y., Yamamoto, T., Takada, S., Matsui, Y., Obinata, M. Antisense RNA of the latent period gene (MER5) inhibits the differentiation of murine erythroleukemia cells. Gene 91: 261-265, 1990. 3. Wonsey, D. R., Zeller, K. I., Dang, C. V. The c-Myc target gene PRDX3 is required for mitochondrial homeostasis and neoplastic transformation. Proc. Nat. Acad. Sci. 99: 6649-6654, 2002. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Peroxiredoxin 3 (PRDX3)
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别名
- PRDX3
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背景
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Synonyms: Thioredoxin-dependent peroxide reductase, mitochondrial,1.11.1.15,Antioxidant protein 1,AOP-1,HBC189,Peroxiredoxin III,Prx-III,Peroxiredoxin-3,Protein MER5 homolog,PRDX3,AOP1,
Tissue Specificity: Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
Background: PRDX3 (peroxiredoxin 3) also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human AOP1 protein shares 86 % amino acid sequence similarity with mouse Aop1, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of PRDX3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that PRDX3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function.
Sequence Similarities: Belongs to the AhpC/TSA family.
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分子量
- 25 kDa
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基因ID
- 10935
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UniProt
- P30048
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途径
- Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process
抗原
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