Peroxiredoxin 1 抗体 (Middle Region)
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- 抗原 See all Peroxiredoxin 1 (PRDX1) 抗体
- Peroxiredoxin 1 (PRDX1)
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抗原表位
- AA 100-128, Middle Region
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Peroxiredoxin 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- 原理
- Anti-Peroxiredoxin 1/PRDX1 Antibody Picoband®
- 序列
- MNIPLVSDPK RTIAQDYGVL KADEGISFR
- 交叉反应 （详细）
- No cross-reactivity with other proteins.
- 产品特性
- Anti-Peroxiredoxin 1/PRDX1 Antibody Picoband® (ABIN3043903). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Peroxiredoxin 1, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.
- 亚型
- IgG
- Top Product
- Discover our top product PRDX1 Primary Antibody
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anti-Peroxiredoxin 1 (PRDX1) antibody
KO Validated PRDX1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Peroxiredoxin 1 (PRDX1) antibodyPRDX1 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 7A1 unconjugated
anti-Peroxiredoxin 1 (PRDX1) (AA 1-100) antibodyPRDX1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peroxiredoxin 1 (PRDX1) (AA 1-199) antibodyPRDX1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Peroxiredoxin 1 (PRDX1) antibodyPRDX1 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 2J5 unconjugated
anti-Peroxiredoxin 1 (PRDX1) (AA 1-199) antibodyPRDX1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B11-D10 unconjugated
anti-Peroxiredoxin 1 (PRDX1) (AA 1-184) antibodyPRDX1 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Peroxiredoxin 1 (PRDX1) (AA 19-116) antibodyPRDX1 适用: 人, 小鼠 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Peroxiredoxin 1 (PRDX1) (Internal Region) antibodyVerified PRDX1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Peroxiredoxin 1 (PRDX1) (AA 88-116) antibodyPRDX1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB20885 unconjugated
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- 应用备注
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Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Neumann, C. A., Krause, D. S., Carman, C. V., Das, S., Dubey, D. P., Abraham, J. L., Bronson, R. T., Fujiwara, Y., Orkin, S. H., Van Etten, R. A.Essential role for the peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumour suppression. Nature 424: 561-565, 2003. 2. Prosperi, M.-T., Apiou, F., Dutrillaux, B., Goubin, G. Organization and chromosomal assignment of two human PAG gene loci: PAGA encoding a functional gene and PAGB a processed pseudogene. Genomics 19: 236-241, 1994. 3. Yan, Y., Sabharwal, P., Rao, M., Sockanathan, S. The antioxidant enzyme Prdx1 controls neuronal differentiation by thiol-redox-dependent activation of GDE2. Cell 138: 1209-1221, 2009. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- 抗原
- Peroxiredoxin 1 (PRDX1)
- 别名
- PRDX1 (PRDX1 产品)
- 背景
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Synonyms: Peroxiredoxin-1,1.11.1.15,Natural killer cell-enhancing factor A,NKEF-A,Proliferation-associated gene protein,PAG,Thioredoxin peroxidase 2,Thioredoxin-dependent peroxide reductase 2,PRDX1,PAGA, PAGB, TDPX2,
Tissue Specificity: Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
Background: PRDX1 (Peroxiredoxin 1), also called PRX1, PAGA or NKEFA, is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The PRDX1 gene is mapped on 1p34.1. Prdx1 was expressed in differentiating motor neuron cells in developing embryonic chicken and mouse spinal cords. mmunoprecipitation analysis showed that GDE2 interacted ly with PRDX1 in embryonic chicken spinal cord extracts and in transfected HEK293T cells. This protein may have a proliferative effect and play a role in cancer development or progression. In differentiating spinal cord, Prdx1 was required to activate Gde2 by reducing an intramolecular cystine bridge between the Gde2 N- and C-terminal domains. An intramolecular disulfide bond between the GDE2 N- and C-terminal domains inhibits GDE2 function, and that reduction of this cystine by PRDX1 activates GDE2 for the induction of motor neuron differentiation.
Sequence Similarities: Belongs to the AhpC/TSA family.
- 分子量
- 24 kDa
- 基因ID
- 5052
- UniProt
- Q06830
- 途径
- p53 Pathway, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
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