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IKZF1 抗体 (C-Term)

This anti-IKZF1 antibody is a 兔 多克隆 antibody detecting IKZF1 in WB, IHC, FACS, IF 和 ICC. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN3043860
发货至: 中国

Quick Overview for IKZF1 抗体 (C-Term) (ABIN3043860)

抗原

See all IKZF1 抗体
IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This IKZF1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 抗原表位

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    AA 428-459, C-Term

    原理

    Anti-Ikaros/IKZF1 Antibody Picoband®

    序列

    LKEEHRAYDL LRAASENSQD ALRVVSTSGE QM

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-Ikaros/IKZF1 Antibody Picoband® (ABIN3043860). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence at the C-terminus of human Ikaros, different from the related mouse sequence by five amino acids.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. Georgopoulos K, Moore DD, Derfler B (December 1992). "Ikaros, an early lymphoid-specific transcription factor and a putative mediator for T cell commitment". Science 258 (5083): 808-12. 2. Hahm K, Ernst P, Lo K, Kim GS, Turck C, Smale ST (November 1994). "The lymphoid transcription factor LyF-1 is encoded by specific, alternatively spliced mRNAs derived from the Ikaros gene". Mol Cell Biol 14 (11): 7111-23.

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

    别名

    IKZF1

    背景

    Synonyms: DNA-binding protein Ikaros,Ikaros family zinc finger protein 1,Lymphoid transcription factor LyF-1,IKZF1,IK1, IKAROS, LYF1, ZNFN1A1,

    Tissue Specificity: Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine. .

    Background: DNA-binding protein Ikaros is a protein that in humans is encoded by the IKZF1 gene. This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors.

    Sequence Similarities: Contains 1 HMG box DNA-binding domain.

    分子量

    50-70 kDa

    基因ID

    10320

    UniProt

    Q13422

    途径

    Production of Molecular Mediator of Immune Response
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