IKZF1 抗体 (C-Term)
Quick Overview for IKZF1 抗体 (C-Term) (ABIN3043860)
抗原
See all IKZF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 428-459, C-Term
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原理
- Anti-Ikaros/IKZF1 Antibody Picoband®
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序列
- LKEEHRAYDL LRAASENSQD ALRVVSTSGE QM
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Ikaros/IKZF1 Antibody Picoband® (ABIN3043860). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human Ikaros, different from the related mouse sequence by five amino acids.
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亚型
- IgG
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anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (AA 1-265) antibody
IKZF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (AA 1-270) antibodyIKZF1 适用: 人 WB, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (AA 415-444), (C-Term) antibodyIKZF1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB24656 unconjugated
anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (C-Term) antibody (PE)IKZF1 适用: 人, 小鼠 ICFC 宿主: 小鼠 Monoclonal 4E9 PE
anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) antibodyIKZF1 适用: 人 WB, IF, ChIP 宿主: 兔 Monoclonal unconjugated
anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (Middle Region) antibodyIKZF1 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (Internal Region) antibodyVerified IKZF1 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (AA 1-477) antibodyIKZF1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (N-Term) antibodyIKZF1 适用: 小鼠 WB, ChIP, ChIP-seq 宿主: 小鼠 Monoclonal 2A9 unconjugated
anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) antibodyIKZF1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Georgopoulos K, Moore DD, Derfler B (December 1992). "Ikaros, an early lymphoid-specific transcription factor and a putative mediator for T cell commitment". Science 258 (5083): 808-12. 2. Hahm K, Ernst P, Lo K, Kim GS, Turck C, Smale ST (November 1994). "The lymphoid transcription factor LyF-1 is encoded by specific, alternatively spliced mRNAs derived from the Ikaros gene". Mol Cell Biol 14 (11): 7111-23. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))
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别名
- IKZF1
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背景
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Synonyms: DNA-binding protein Ikaros,Ikaros family zinc finger protein 1,Lymphoid transcription factor LyF-1,IKZF1,IK1, IKAROS, LYF1, ZNFN1A1,
Tissue Specificity: Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine. .
Background: DNA-binding protein Ikaros is a protein that in humans is encoded by the IKZF1 gene. This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors.
Sequence Similarities: Contains 1 HMG box DNA-binding domain.
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分子量
- 50-70 kDa
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基因ID
- 10320
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UniProt
- Q13422
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途径
- Production of Molecular Mediator of Immune Response
抗原
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