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IKZF1 抗体 (N-Term)

There is 1 publication for this product available. The 小鼠 单克隆 anti-IKZF1 antibody is suitable to detect IKZF1 in samples from 小鼠. It has been validated for WB, ChIP 和 ChIP-seq.
产品编号 ABIN2668828
发货至: 中国
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Quick Overview for IKZF1 抗体 (N-Term) (ABIN2668828)

抗原

See all IKZF1 抗体
IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

适用

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小鼠

宿主

  • 80
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小鼠

克隆类型

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单克隆

标记

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This IKZF1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

克隆位点

2A9
  • 抗原表位

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    N-Term

    原理

    Ikaros antibody (mAb) (Clone 2A9)

    纯化方法

    Protein G Chromatography

    免疫原

    This Ikaros antibody was raised against a recombinant protein corresponding to the N-terminal half of mouse Ikaros.

    亚型

    IgG
  • 应用备注

    ChIP: 4 μg per ChIP ChIP-Seq: 4 μg each WB: 0.5 - 2 μg/mL dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here.

    限制

    仅限研究用
  • 浓度

    1 μg/μL

    缓冲液

    Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

    储存条件

    -20 °C

    储存方法

    Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

    有效期

    24 months
  • Yeung, Chung, Guess, Bell, Leinwand: "Myh7b/miR-499 gene expression is transcriptionally regulated by MRFs and Eos." in: Nucleic acids research, Vol. 40, Issue 15, pp. 7303-18, (2012) (PubMed).

  • 抗原

    IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

    别名

    Ikaros

    背景

    Ikaros is a transcription factor that plays a critical role in the control of lymphohematopoiesis and immune regulation. All Ikaros isoforms share a common C-terminal domain that contains a transcription activation motif and two zinc finger motifs required for hetero- and homo-dimerization among the Ikaros isoforms, and for interactions with other proteins. Ikaros is abundantly expressed in pituitary mammosomatotrophs, where it deacetylates Histone H3 sites on the proximal growth hormone (GH) promoter to silence gene expression.

    分子量

    62 kDa

    基因ID

    22778

    NCBI登录号

    NM_001025597

    途径

    Production of Molecular Mediator of Immune Response
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