CDC25B 抗体 (AA 119-248)
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- 抗原 See all CDC25B 抗体
- CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
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抗原表位
- AA 119-248
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适用
- 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CDC25B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for M-phase inducer phosphatase 2(CDC25B) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 交叉反应 （详细）
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Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
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Rabbit IgG polyclonal antibody for M-phase inducer phosphatase 2(CDC25B) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: cell division cycle 25 homolog B (S. pombe)
Protein Name: M-phase inducer phosphatase 2 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- E.coli-derived human Cdc25B recombinant protein (Position: M119-L248). Human Cdc25B shares 71% and 68.2% amino acid (aa) sequence identity with mouse and rat Cdc25B, respectively.
- 亚型
- IgG
- Top Product
- Discover our top product CDC25B Primary Antibody
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anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer186) antibody
CDC25B 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibodyCDC25B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibodyCDC25B 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer149) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 1-195) antibodyCDC25B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 331-360) antibodyCDC25B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB07936 unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer149) antibodyCDC25B 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) antibodyCDC25B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
- 别名
- CDC25B (CDC25B 产品)
- 别名
- CDC25B antibody, AI604853 antibody, cell division cycle 25B antibody, cdc25b antibody, CDC25B antibody, Cdc25b antibody
- 背景
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Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human CDC25 genes code for the A, B, and C forms of CDC25. CDC25B is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216, phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
Synonyms: AI604853 antibody|Cdc 25B antibody|Cdc25b antibody|CDC25B1 antibody|CDC25B2 antibody| CDC25B3 antibody|CDC25HU2 antibody|Cdc25m2 antibody|Cell division cycle 25 homolog B antibody|Cell division cycle 25B antibody|Cell division cycle 25B isoform 1 antibody|Cell division cycle 25B isoform 2 antibody|Cell division cycle 25B isoform 3 antibody|Cell division cycle 25B isoform 4 antibody|Dual specificity phosphatase Cdc25B antibody|EC 3.1.3.48 antibody|M phase inducer phosphatase 2 antibody|M-phase inducer phosphatase 2 antibody|MPIP2_HUMAN antibody - 基因ID
- 994
- UniProt
- P30305
- 途径
- Cell Division Cycle, M Phase, Autophagy
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